Publications - Hung - 2015

  • Avraham R, Haseley N, Brown D, Penaranda C, Jijon HB, Trombetta JJ, Satija R, Shalek AK, Xavier RJ, Regev A, Hung DT

    Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses. Cell. 2015;162(6):1309-21 - PMID: 26343579 - PMCID: PMC4578813 - DOI: 10.1016/j.cell.2015.08.027

  • Avraham R, Haseley N, Brown D, Penaranda C, Jijon HB, Trombetta JJ, Satija R, Shalek AK, Xavier RJ, Regev A, Hung DT

    Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses. Cell. 2015;162(6):1309-21 - PMID: 26343579 - PMCID: PMC4578813 - DOI: 10.1016/j.cell.2015.08.027

  • Hou HW, Bhattacharyya RP, Hung DT, Han J

    Direct detection and drug-resistance profiling of bacteremias using inertial microfluidics. Lab Chip. 2015;15(10):2297-2307 - PMID: 25882432 - PMCID: PMC4437799 - DOI: 10.1039/c5lc00311c

  • Shishkin AA, Giannoukos G, Kucukural A, Ciulla D, Busby M, Surka C, Chen J, Bhattacharyya RP, Rudy RF, Patel MM, Novod N, Hung DT, Gnirke A, Garber M, Guttman M, Livny J

    Simultaneous generation of many RNA-seq libraries in a single reaction. Nat Methods. 2015;12(4):323-325 - PMID: 25730492 - PMCID: PMC4712044 - DOI: 10.1038/nmeth.3313

  • Wivagg CN, Bhattacharyya RP, Hung DT

    Mechanisms of ?-lactam killing and resistance in the context of Mycobacterium tuberculosis. J Antibiot (Tokyo). 2014;67(9):645-54 - PMID: 25052484 - DOI: 10.1038/ja.2014.94

  • Stanley SA, Barczak AK, Silvis MR, Luo SS, Sogi K, Vokes M, Bray MA, Carpenter AE, Moore CB, Siddiqi N, Rubin EJ, Hung DT

    Identification of Host-Targeted Small Molecules That Restrict Intracellular Mycobacterium tuberculosis Growth. PLoS Pathog. 2014;10(2):e1003946 - PMID: 24586159 - PMCID: PMC3930586 - DOI: 10.1371/journal.ppat.1003946

  • Slater LH, Clatworthy AE, Hung DT

    Bacterial toxins and small molecules elucidate endosomal trafficking. Trends Microbiol. 2014;22(2):53-5 - PMID: 24370463 - DOI: 10.1016/j.tim.2013.12.002

  • Wright GD, Hung DT, Helmann JD

    How antibiotics kill bacteria: new models needed?. Nat Med. 2013;19(5):544-5 - PMID: 23652106 - DOI: 10.1038/nm.3198

  • Kawate T, Iwase N, Shimizu M, Stanley SA, Wellington S, Kazyanskaya E, Hung DT

    Synthesis and structure-activity relationships of phenyl-substituted coumarins with anti-tubercular activity that target FadD32. Bioorg Med Chem Lett. 2013;23(22):6052-9 - PMID: 24103299 - DOI: 10.1016/j.bmcl.2013.09.035

  • Grant SS, Kawate T, Nag PP, Silvis MR, Gordon K, Stanley SA, Kazyanskaya E, Nietupski R, Golas A, Fitzgerald M, Cho S, Franzblau SG, Hung DT

    Identification of novel inhibitors of non-replicating M. tuberculosis using a carbon starvation model. ACS Chem Biol. 2013;8(10):2224-34 - PMID: 23898841 - PMCID: PMC3864639 - DOI: 10.1021/cb4004817

  • Stanley SA, Kawate T, Iwase N, Shimizu M, Clatworthy AE, Kazyanskaya E, Sacchettini JC, Ioerger TR, Siddiqi NA, Minami S, Aquadro JA, Schmidt Grant S, Rubin EJ, Hung DT

    Diarylcoumarins inhibit mycolic acid biosynthesis and kill Mycobacterium tuberculosis by targeting FadD32. Proc Natl Acad Sci U S A. 2013;110(28):11565-70 - PMID: 23798446 - PMCID: PMC3710825 - DOI: 10.1073/pnas.1302114110

  • Hett EC, Slater LH, Mark KG, Kawate T, Monks BG, Stutz A, Latz E, Hung DT

    Chemical genetics reveals a kinase-independent role for protein kinase R in pyroptosis. Nat Chem Biol. 2013;9(7):466 - PMID: 23774875

  • Slater LH, Hett EC, Clatworthy AE, Mark KG, Hung DT

    CCT chaperonin complex is required for efficient delivery of anthrax toxin into the cytosol of host cells. Proc Natl Acad Sci U S A. 2013;110(24):9932-7 - PMID: 23716698 - PMCID: PMC3683768 - DOI: 10.1073/pnas.1302257110

  • Grant SS, Hung DT

    Persistent bacterial infections, antibiotic tolerance, and the oxidative stress response. Virulence. 2013;4(4):273-283 - PMID: 23563389 - PMCID: PMC3710330 - DOI: 10.4161/viru.23987

  • Hett EC, Slater LH, Mark KG, Kawate T, Monks BG, Stutz A, Latz E, Hung DT

    Chemical genetics reveals a kinase-independent role for protein kinase R in pyroptosis. Nat Chem Biol. 2013;9(6):398-405 - PMID: 23603659 - PMCID: PMC6615456 - DOI: 10.1038/nchembio.1236

  • Slater LH, Hett EC, Mark K, Chumbler NM, Patel D, Lacy DB, Collier RJ, Hung DT

    Identification of Novel Host-Targeted Compounds That Protect From Anthrax Lethal Toxin-Induced Cell Death. ACS Chem Biol. 2013;8(4):812-822 - PMID: 23343607 - PMCID: PMC3638717 - DOI: 10.1021/cb300555n

  • Grad YH, Lipsitch M, Griggs AD, Haas BJ, Shea TP, McCowan C, Montmayeur A, FitzGerald M, Wortman JR, Krogfelt KA, Bingen E, Weill FX, Tietze E, Flieger A, Lander ES, Nusbaum C, Birren BW, Hung DT, Hanage WP

    Reply to Guy et al.: Support for a bottleneck in the 2011 Escherichia coli O104:H4 outbreak in Germany. Proc Natl Acad Sci U S A. 2012;109(52):E3629-30 - PMID: 23479789 - PMCID: PMC3535640 - DOI: 10.1073/pnas.1209419110

  • Chand NS, Clatworthy AE, Hung DT

    The Two-Component Sensor KinB Acts as a Phosphatase to Regulate Pseudomonas aeruginosa Virulence. J Bacteriol. 2012;194(23):6537-47 - PMID: 23024348 - PMCID: PMC3497526 - DOI: 10.1128/JB.01168-12

  • Schmidt Grant S, Kaufmann BB, Chand NS, Haseley N, Hung DT

    Eradication of bacterial persisters with antibiotic-generated hydroxyl radicals. Proc Natl Acad Sci U S A. 2012;109(30):12147-52 - PMID: 22778419 - PMCID: PMC3409745 - DOI: 10.1073/pnas.1203735109

  • Stanley SA, Schmidt Grant S, Kawate T, Iwase N, Shimizu M, Wivagg C, Silvis M, Kazyanskaya E, Aquadro J, Golas A, Fitzgerald M, Dai H, Zhang L, Hung DT

    Identification of novel inhibitors of M. tuberculosis growth using whole cell based high-throughput screening. ACS Chem Biol. 2012;7(8):1377-84 - PMID: 22577943 - PMCID: PMC3560293 - DOI: 10.1021/cb300151m

  • Grad YH, Lipsitch M, Feldgarden M, Arachchi HM, Cerqueira GC, Fitzgerald M, Godfrey P, Haas BJ, Murphy CI, Russ C, Sykes S, Walker BJ, Wortman JR, Young S, Zeng Q, Abouelleil A, Bochicchio J, Chauvin S, Desmet T, Gujja S, McCowan C, Montmayeur A, Steelman S, Frimodt-Møller J, Petersen AM, Struve C, Krogfelt KA, Bingen E, Weill FX, Lander ES, Nusbaum C, Birren BW, Hung DT, Hanage WP

    Genomic epidemiology of the Escherichia coli O104:H4 outbreaks in Europe, 2011. Proc Natl Acad Sci U S A. 2012;109(8):3065-70 - PMID: 22315421 - PMCID: PMC3286951 - DOI: 10.1073/pnas.1121491109

  • Barczak AK, Gomez JE, Kaufmann BB, Hinson ER, Cosimi L, Borowsky ML, Onderdonk AB, Stanley SA, Kaur D, Bryant KF, Knipe DM, Sloutsky A, Hung DT

    RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities. Proc Natl Acad Sci U S A. 2012;109(16):6217-22 - PMID: 22474362 - PMCID: PMC3341018 - DOI: 10.1073/pnas.1119540109

  • Gomez JE, Clatworthy A, Hung DT

    Probing bacterial pathogenesis with genetics, genomics, and chemical biology: past, present, and future approaches. Crit Rev Biochem Mol Biol. 2011;46(1):41-66 - PMID: 21250782 - DOI: 10.3109/10409238.2010.538663

  • Chand NS, Lee JS, Clatworthy AE, Golas AJ, Smith RS, Hung DT

    The sensor kinase KinB regulates virulence in acute Pseudomonas aeruginosa infection. J Bacteriol. 2011;193(12):2989-99 - PMID: 21515773 - PMCID: PMC3133189 - DOI: 10.1128/JB.01546-10

  • Tamayo AG, Slater L, Taylor-Parker J, Bharti A, Harrison R, Hung DT, Murphy JR

    GRP78(BiP) facilitates the cytosolic delivery of anthrax lethal factor (LF) in vivo and functions as an unfoldase in vitro. Mol Microbiol. 2011;81(5):1390-401 - PMID: 21797942 - PMCID: PMC4041151 - DOI: 10.1111/j.1365-2958.2011.07770.x

  • Chand NS, Hung DT

    The two-component sensor kinase KinB acts as a non-canonical switch between acute and chronic infection. Virulence. 2011;2(6):553-8 - PMID: 21971182 - DOI: 10.4161/viru.2.6.17987

  • Kaufmann BB, Hung DT

    The fast track to multidrug resistance. Mol Cell. 2010;37(3):297-8 - PMID: 20159549 - DOI: 10.1016/j.molcel.2010.01.027

  • Clatworthy AE, Lee JS, Leibman M, Kostun Z, Davidson AJ, Hung DT

    Pseudomonas aeruginosa infection of zebrafish involves both host and pathogen determinants. Infect Immun. 2009;77(4):1293-303 - PMID: 19168742 - PMCID: PMC2663173 - DOI: 10.1128/IAI.01181-08

  • Tran C, Ouk S, Clegg NJ, Chen Y, Watson PA, Arora V, Wongvipat J, Smith-Jones PM, Yoo D, Kwon A, Wasielewska T, Welsbie D, Chen CD, Higano CS, Beer TM, Hung DT, Scher HI, Jung ME, Sawyers CL

    Development of a second-generation antiandrogen for treatment of advanced prostate cancer. Science. 2009;324(5928):787-90 - PMID: 19359544 - PMCID: PMC2981508 - DOI: 10.1126/science.1168175

  • Barczak AK, Hung DT

    Productive steps toward an antimicrobial targeting virulence. Curr Opin Microbiol. 2009;12(5):490-6 - PMID: 19631578 - PMCID: PMC2763366 - DOI: 10.1016/j.mib.2009.06.012

  • Stanley SA, Hung DT

    Chemical tools for dissecting bacterial physiology and virulence. Biochemistry. 2009;48(37):8776-86 - PMID: 19653697 - DOI: 10.1021/bi9009083

  • Hett EC, Hung DT

    Targeting multiple biofilm pathways. Chem Biol. 2009;16(12):1216-8 - PMID: 20064430 - DOI: 10.1016/j.chembiol.2009.12.003

  • Stanley SA, Hung DT

    Toward a systems-level analysis of infection biology: a new method for conducting genetic screens in human cells. Sci Transl Med. 2009;1(11):11ps13 - PMID: 20371455 - DOI: 10.1126/scitranslmed.3000550

  • Shakhnovich EA, Hung DT, Pierson E, Lee K, Mekalanos JJ

    Virstatin inhibits dimerization of the transcriptional activator ToxT. Proc Natl Acad Sci U S A. 2007;104(7):2372-7 - PMID: 17283330 - PMCID: PMC1892951 - DOI: 10.1073/pnas.0611643104

  • Clatworthy AE, Pierson E, Hung DT

    Targeting virulence: a new paradigm for antimicrobial therapy. Nat Chem Biol. 2007;3(9):541-8 - PMID: 17710100 - DOI: 10.1038/nchembio.2007.24

  • Hung DT, Zhu J, Sturtevant D, Mekalanos JJ

    Bile acids stimulate biofilm formation in Vibrio cholerae. Mol Microbiol. 2006;59(1):193-201 - PMID: 16359328 - DOI: 10.1111/j.1365-2958.2005.04846.x

  • Hung DT, Rubin EJ

    Chemical biology and bacteria: not simply a matter of life or death. Curr Opin Chem Biol. 2006;10(4):321-6 - PMID: 16814591 - DOI: 10.1016/j.cbpa.2006.06.016

  • Hung DT, Mekalanos JJ

    Bile acids induce cholera toxin expression in Vibrio cholerae in a ToxT-independent manner. Proc Natl Acad Sci U S A. 2005;102(8):3028-33 - PMID: 15699331 - PMCID: PMC549475 - DOI: 10.1073/pnas.0409559102

  • Hung DT, Shakhnovich EA, Pierson E, Mekalanos JJ

    Small-molecule inhibitor of Vibrio cholerae virulence and intestinal colonization. Science. 2005;310(5748):670-4 - PMID: 16223984 - DOI: 10.1126/science.1116739

  • O'Shaughnessy JA, Ljung BM, Dooley WC, Chang J, Kuerer HM, Hung DT, Grant MD, Khan SA, Phillips RF, Duvall K, Euhus DM, King BL, Anderson BO, Troyan SL, Kim J, Veronesi U, Cazzaniga M

    Ductal lavage and the clinical management of women at high risk for breast carcinoma: a commentary. Cancer. 2002;94(2):292-8 - PMID: 11900214 - DOI: 10.1002/cncr.10238

  • Evron E, Dooley WC, Umbricht CB, Rosenthal D, Sacchi N, Gabrielson E, Soito AB, Hung DT, Ljung B, Davidson NE, Sukumar S

    Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR. Lancet. 2001;357(9265):1335-6 - PMID: 11343741 - DOI: 10.1016/s0140-6736(00)04501-3

  • Dooley WC, Ljung BM, Veronesi U, Cazzaniga M, Elledge RM, O'Shaughnessy JA, Kuerer HM, Hung DT, Khan SA, Phillips RF, Ganz PA, Euhus DM, Esserman LJ, Haffty BG, King BL, Kelley MC, Anderson MM, Schmit PJ, Clark RR, Kass FC, Anderson BO, Troyan SL, Arias RD, Quiring JN, Love SM, Page DL, King EB

    Ductal lavage for detection of cellular atypia in women at high risk for breast cancer. J Natl Cancer Inst. 2001;93(21):1624-32 - PMID: 11698566 - DOI: 10.1093/jnci/93.21.1624

  • Hung DT, Chen J, Schreiber SL

    (+)-Discodermolide binds to microtubules in stoichiometric ratio to tubulin dimers, blocks taxol binding and results in mitotic arrest. Chem Biol. 1996;3(4):287-93 - PMID: 8807856 - DOI: 10.1016/s1074-5521(96)90108-8

  • Hung DT, Jamison TF, Schreiber SL

    Understanding and controlling the cell cycle with natural products. Chem Biol. 1996;3(8):623-39 - PMID: 8807895 - DOI: 10.1016/s1074-5521(96)90129-5

  • Hung DT, Nerenberg JB, Schreiber SL

    Distinct binding and cellular properties of synthetic (+)- and (-)-discodermolides. Chem Biol. 1994;1(1):67-71 - PMID: 9383372 - DOI: 10.1016/1074-5521(94)90042-6

  • Coughlin SR, Scarborough RM, Vu TK, Hung DT

    Thrombin receptor structure and function. Cold Spring Harb Symp Quant Biol. 1993;57:149-54 - PMID: 1339654 - DOI: 10.1101/sqb.1992.057.01.019

  • Bram RJ, Hung DT, Martin PK, Schreiber SL, Crabtree GR

    Identification of the immunophilins capable of mediating inhibition of signal transduction by cyclosporin A and FK506: roles of calcineurin binding and cellular location. Mol Cell Biol. 1993;13(8):4760-9 - PMID: 7687744 - PMCID: PMC360102 - DOI: 10.1128/mcb.13.8.4760

  • Coughlin SR, Vu TK, Hung DT, Wheaton VI

    Expression cloning and characterization of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Semin Thromb Hemost. 1992;18(2):161-6 - PMID: 1321504 - DOI: 10.1055/s-2007-1002422

  • Hung DT, Vu TH, Nelken NA, Coughlin SR

    Thrombin-induced events in non-platelet cells are mediated by the unique proteolytic mechanism established for the cloned platelet thrombin receptor. J Cell Biol. 1992;116(3):827-32 - PMID: 1309820 - PMCID: PMC2289317 - DOI: 10.1083/jcb.116.3.827

  • Coughlin SR, Vu TK, Hung DT, Wheaton VI

    Characterization of a functional thrombin receptor. Issues and opportunities. J Clin Invest. 1992;89(2):351-5 - PMID: 1310691 - PMCID: PMC442859 - DOI: 10.1172/JCI115592

  • Hung DT, Vu TK, Wheaton VI, Charo IF, Nelken NA, Esmon N, Esmon CT, Coughlin SR

    "Mirror image" antagonists of thrombin-induced platelet activation based on thrombin receptor structure. J Clin Invest. 1992;89(2):444-50 - PMID: 1310695 - PMCID: PMC442871 - DOI: 10.1172/JCI115604

  • Hung DT, Vu TK, Wheaton VI, Ishii K, Coughlin SR

    Cloned platelet thrombin receptor is necessary for thrombin-induced platelet activation. J Clin Invest. 1992;89(4):1350-3 - PMID: 1313452 - PMCID: PMC442997 - DOI: 10.1172/JCI115721

  • Garboczi DN, Hung DT, Wiley DC

    HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides. Proc Natl Acad Sci U S A. 1992;89(8):3429-33 - PMID: 1565634 - PMCID: PMC48881 - DOI: 10.1073/pnas.89.8.3429

  • Hung DT, Schreiber SL

    cDNA cloning of a human 25 kDa FK506 and rapamycin binding protein. Biochem Biophys Res Commun. 1992;184(2):733-8 - PMID: 1374240 - DOI: 10.1016/0006-291x(92)90651-z

  • Scarborough RM, Naughton MA, Teng W, Hung DT, Rose J, Vu TK, Wheaton VI, Turck CW, Coughlin SR

    Tethered ligand agonist peptides. Structural requirements for thrombin receptor activation reveal mechanism of proteolytic unmasking of agonist function. J Biol Chem. 1992;267(19):13146-9 - PMID: 1320011

  • Hung DT, Wong YH, Vu TK, Coughlin SR

    The cloned platelet thrombin receptor couples to at least two distinct effectors to stimulate phosphoinositide hydrolysis and inhibit adenylyl cyclase. J Biol Chem. 1992;267(29):20831-4 - PMID: 1328213

  • Vu TK, Hung DT, Wheaton VI, Coughlin SR

    Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell. 1991;64(6):1057-68 - PMID: 1672265 - DOI: 10.1016/0092-8674(91)90261-v

  • Vu TK, Wheaton VI, Hung DT, Charo I, Coughlin SR

    Domains specifying thrombin-receptor interaction. Nature. 1991;353(6345):674-7 - PMID: 1717851 - DOI: 10.1038/353674a0

  • Hung DT, Oredsson SM, Pegg AE, Deen DF, Marton LJ

    Potentiation of 1,3-bis(2-chloroethyl)-1-nitrosourea cytotoxicity in 9L rat brain tumor cells by methylglyoxal-bis(guanylhydrazone), an inhibitor of S-adenosyl-L-methionine decarboxylase. Eur J Cancer Clin Oncol. 1984;20(3):417-20 - PMID: 6538500 - DOI: 10.1016/0277-5379(84)90090-7

  • Alhonen-Hongisto L, Hung DT, Deen DF, Marton LJ

    Decreased cell kill of vincristine and methotrexate against 9L rat brain tumor cells in vitro caused by alpha-difluoromethylornithine-induced polyamine depletion. Cancer Res. 1984;44(10):4440-2 - PMID: 6432313

  • Hung DT, Marton LJ, Deen DF, Shafer RH

    Depletion of intracellular polyamines may alter DNA conformation in 9L rat brain tumor cells. Science. 1983;221(4608):368-70 - PMID: 6408733 - DOI: 10.1126/science.6408733

  • Hung DT, Deen DF, Seidenfeld J, Marton LJ

    Sensitization of 9L rat brain gliosarcoma cells to 1,3-bis(2-chloroethyl)-1-nitrosourea by alpha-difluoromethylornithine, an ornithine decarboxylase inhibitor. Cancer Res. 1981;41(7):2783-5 - PMID: 6788362

  • Hung DT, Benner SA, Williams CM

    Preparation of an ecdysone immunogen for radioimmunoassay work. J Biol Chem. 1980;255(13):6047-8 - PMID: 7190146