DNA Core

The CCIB DNA Core, founded in 1995, is a well-established major research core laboratory within the Center for Computational and Integrative Biology of Massachusetts General Hospital Boston and has expertise in genomics, molecular biology, and laboratory automation. The Core provides cost-effective, high-quality services for investigators of the MGH/Partners research community as well as for clients from non-profit organizations and industry throughout the United States and across the world.

Our mission is to accelerate fundamental scientific discoveries that will improve human health. We are constantly striving to advance research productivity by reliably generating the highest quality data with rapid turn-around time and competitive pricing, and by working with our customers on an individual basis to address their specific research needs. Realizing the constant changes of our customers’ research needs, we make every effort to continuously improve our services and to offer new and innovative solutions.

Research Services include:

  • Sanger DNA Sequencing
  • DNA Fragment Analyis (Microsatellite)
  • Complete Plasmid Sequencing — NEW!
  • CRISPR Sequencing — NEW!
  • DNA Oligonucleotide Synthesis
  • 96-well Plasmid Preparation
  • Mouse Genotyping
  • High-Throughput cDNA Library Screening — NEW!
  • High-Throughput siRNA Library Sreening
  • Bacterial Colony Picking & Archiving
  • Automated Molecular Biology Sample Handling
  • Custom Automation Projects

For detailed information about the core facility’s current services and capabilities, please visit our website.

If you’d like to discuss your project, schedule an introductory meeting or a consultation, or request a cost estimate or quote, please contact the Core Director:

Nicole Stange-Thomann, Ph.D.
Phone: 617-726-0085
Email

DNA Core Team

Karen Armstrong (Elmassadi)
Research Technologist
Amy Avery
DNA Core Director
Julian Banerji
Visiting Scientist
Kristina Belanger
Senior Research Technologist
Stephen Belmonte
Sr. Research Technologist, Sequencing
Marc Davignon
Systems Manager
Aissatou Diallo
Research Technician II
Michael Mirisola
Research Technician II, Automation
Andrew Moeller
Research Technologist, Synthesis
Terry Orechia
Web Developer
Huajun Wang
Bioinformatics Specialist II

DNA Core Careers

Program: DNA Core
Date Posted: 2017-05-30
Description
The CCIB DNA Core has opened up a full-time position for a Research Technologist. As a member of the DNA Core, the ResearchTechnologist will provide standard and custom medium- to high-throughput sample processing services for various molecular biology and genomics applications, including maintenance of automated laboratory workstations, and will participate in the development, implementation and execution of automated molecular biology procedures and processes. The Research Technologist reports directly to Core Director, and/or managers of the core laboratory and is expected to work independently on assigned research with minimal guidance from a higher level staff member. The ideal candidate must be able to learn and work in a collaborative and fast-paced team environment, and to interact with all levels of staff and with researchers using our services. He/she will have good organizational and time management skills as well as an ability both to follow directions carefully and to solve common problems independently. We are looking for a team member who can learn quickly and is enthusiastic about laboratory automation instruments and devices and methodologies used to enable, expedite and increase the efficiency and effectiveness of scientific research. Review of applications will begin immediately and continue until the position is filled. Salary will be commensurate with the candidate’s experience and qualifications. The position typically requires a bachelor’s degree or higher plus three years of related experience, or an equivalent combination of education and experience.
Responsibilities
Develops, validates, and applies high-throughput methods for various scientific applications using and programming laboratory automation. Provides standard and custom medium- to high-throughput sample processing services for molecular biology and genomics applications (including but not limited to Custom Automation projects, Mouse Genotyping, 96-well Plasmid Preparation, High-Throughput Library Screening, and Next-Generation Sequencing (NGS) Library Preparation). Working with senior personnel, performs early research and development activity to create new core services that are robust, cost-effective and time efficient. Collaborates with core customers (senior scientists and postdoctoral research fellows) to transfer new assays and methods to automation platforms. Works closely with IT group and instrument vendors to develop and implement integration layers between automation platform and the LIMS systems. Develops project timelines and utilizes time management practices and tools to manage demands and deadlines and to deliver on key project milestones; proactively communicates impending changes in work, project schedules and experimental design. Accurately and consistently records experimental methods, materials and results in laboratory notebooks. Analyzes data, evaluates results, forms conclusions, and determines future experiments. Uses broad knowledge of molecular biology techniques to proactively troubleshoot assays, to resolve technical issues and challenges, and to perform data interpretation. Presents findings or comprehensive project status reviews at internal meetings. Applies advanced technical writing and graphical skills to produce documents and reports. Writes and implements standard operation procedures (SOPs). Determines all chemicals, reagents and supplies needed for a project; develops a strong knowledge of all related third-party vendors and their technologies and products. Maintains and troubleshoots liquid handling platforms, including cleanliness of work areas and instrument scheduling for various core and collaborator activities. Assists Core Director in cost modeling and in the analysis of yearly volumes of automation services for the core budget process. Flexibility in a fast-paced, changing environment; be willing to accept responsibilities outside of the initial job description. Establishes and maintains productive relationship with all core personnel; develops internal and external collaborations. For specific research services, works as a liaison between core laboratory and customers.
Requirements
Strong molecular biology background and good hands-on knowledge of the latest molecular biology methods Experience operating and programming liquid-handling robotics Proficiency in Microsoft Word, Excel, and PowerPoint Excellent general laboratory and organizational skills including the ability to multitask and to work independently and well as part of a team Superior time management skills Excellent communication skills (written and verbal) and orientation toward teamwork and collaboration Critical thinking, excellent data analysis and interpretation skills, good problem solving skills Preferred: Background in automated sample processing Ability to operate LIMS Experience in: Laboratory Automation/Liquid Handling; High-throughput sample processing; cDNA screening; Mouse Genotyping; Next-Generation sequencing methods and library preparation; Real-time PCR; Advanced molecular biology techniques used in Genomics Next-Generation Sequencing using Illumina instruments such as MiSeq Education: The candidate must have a bachelor’s degree or higher in the Life Sciences, with strong training in the theory and practice of molecular biology, plus a minimum of three years experience in a research environment (5 years preferred), or an equivalent combination of education and experience. Specific experience in programming and use of laboratory robotics for high-throughput sample processing is required. LIMS experience and/or familiarity with some of the technologies listed under Responsibilities is a plus. Some related work experience in a fast-paced and multi-tasked environment is preferred.