Copying of mixed sequence RNA templates inside model protocells.

O'Flaherty DK, Kamat NP, Mirza FN, Li L, Prywes N, Szostak JW
J Am Chem Soc. 2018;140(15):5171-5178 - PMID: 29608310 - DOI: 10.1021/jacs.8b00639

Fatty Acid/Phospholipid Blended Membranes: A Potential Intermediate State in Protocellular Evolution.

Jin L, Kamat NP, Jena S, Szostak JW
Small. 2018;14(15):e1704077 - PMID: 29479815 - DOI: 10.1002/smll.201704077

Preparation, Purification, and Use of Fatty Acid-containing Liposomes.

Jin L, Engelhart AE, Adamala KP, Szostak JW
J Vis Exp. 2018;(132):ePub - PMID: 29553563 - PMCID: PMC5912384 - DOI: 10.3791/57324

A structural rationale for the enhanced catalysis of nonenzymatic RNA primer extension by a downstream oligonucleotide.

Zhang W, Tam CP, Zhou L, Oh SS, Wang J, Szostak JW
J Am Chem Soc. 2018;140(8):2829-2840 - PMID: 29411978 - DOI: 10.1021/jacs.7b11750

Solvated-electron production using cyanocuprates is compatible with the UV-environment on a Hadean-Archaean Earth.

Todd ZR, Fahrenbach AC, Magnani CJ, Ranjan S, Björkbom A, Szostak JW, Sasselov DD
Chem Commun (Camb). 2018;54(9):1121-1124 - PMID: 29334083 - DOI: 10.1039/c7cc07748c

Synthesis of a nonhydrolyzable nucleotide phosphoroimidazolide analogue that catalyzes nonenzymatic RNA primer extension.

Tam CP, Zhou L, Fahrenbach AC, Zhang W, Walton T, Szostak JW
J Am Chem Soc. 2017;140(2):783-792 - PMID: 29251930 - DOI: 10.1021/jacs.7b11623

A Mechanistic Explanation for the Regioselectivity of Nonenzymatic RNA Primer Extension.

Giurgiu C, Li L, O'Flaherty DK, Tam CP, Szostak JW
J Am Chem Soc. 2017;139(46):16741-16747 - PMID: 29112424 - DOI: 10.1021/jacs.7b08784

A kinetic model of nonenzymatic RNA polymerization by cytidine-5'-phosphoro-2-aminoimidazolide.

Walton T, Szostak JW
Biochemistry. 2017;56(43):5739-5747 - PMID: 29022704 - DOI: 10.1021/acs.biochem.7b00792

UV-light-driven prebiotic synthesis of iron-sulfur clusters.

Bonfio C, Valer L, Scintilla S, Shah S, Evans DJ, Jin L, Szostak JW, Sasselov DD, Sutherland JD, Mansy SS
Nat Chem. 2017;9(12):1229-1234 - PMID: 29168482 - PMCID: PMC5808832 - DOI: 10.1038/nchem.2817

Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex.

Zhang W, Tam CP, Walton T, Fahrenbach AC, Birrane G, Szostak JW
Proc Natl Acad Sci U S A. 2017;114(29):7659-7664 - PMID: 28673998 - PMCID: PMC5530681 - DOI: 10.1073/pnas.1704006114

Common and Potentially Prebiotic Origin for Precursors of Nucleotide Synthesis and Activation.

Fahrenbach AC, Giurgiu C, Tam CP, Li L, Hongo Y, Aono M, Szostak JW
J Am Chem Soc. 2017;139(26):8780-8783 - PMID: 28640999 - DOI: 10.1021/jacs.7b01562

Divergent prebiotic synthesis of pyrimidine and 8-oxo-purine ribonucleotides.

Stairs S, Nikmal A, Bucar DK, Zheng SL, Szostak JW, Powner MW
Nat Commun. 2017;8:15270 - PMID: 28524845 - PMCID: PMC5454461 - DOI: 10.1038/ncomms15270

The Narrow Road to the Deep Past.

Szostak JW
Angew Chem Int Ed Engl. 2017;56(37):11037-11043 - PMID: 28514493 - DOI: 10.1002/anie.201704048

Enhanced Nonenzymatic RNA Copying with 2-Aminoimidazole Activated Nucleotides.

Li L, Prywes N, Tam CP, O'Flaherty DK, Lelyveld VS, Izgu EC, Pal A, Szostak JW
J Am Chem Soc. 2017;139(5):1810-1813 - PMID: 28117989 - DOI: 10.1021/jacs.6b13148

Downstream Oligonucleotides Strongly Enhance the Affinity of GMP to RNA Primer-Template Complexes.

Tam CP, Fahrenbach AC, Björkbom A, Prywes N, Izgu EC, Szostak JW
J Am Chem Soc. 2017;139(2):571-574 - PMID: 28055190 - DOI: 10.1021/jacs.6b09760

N-Carboxyanhydride-Mediated Fatty Acylation of Amino Acids and Peptides for Functionalization of Protocell Membranes.

Izgu EC, Björkbom A, Kamat NP, Lelyveld VS, Zhang W, Jia TZ, Szostak JW
J Am Chem Soc. 2016;138(51):16669-16676 - PMID: 27959544 - DOI: 10.1021/jacs.6b08801

Unusual Base-Pairing Interactions in Monomer-Template Complexes.

Zhang W, Tam CP, Wang J, Szostak JW
ACS Cent Sci. 2016;2(12):916-926 - PMID: 28058281 - PMCID: PMC5200924 - DOI: 10.1021/acscentsci.6b00278

Effect of terminal 3'-hydroxymethyl modification of an RNA primer on nonenzymatic primer extension.

Pal A, Das RS, Zhang W, Lang M, McLaughlin LW, Szostak JW
Chem Commun (Camb). 2016;52(80):11905-11907 - PMID: 27722518 - DOI: 10.1039/c6cc06925h

On the origin of life.

Szostak JW
Medicina (B Aires). 2016;76(4):199-203 - PMID: 27576276

A highly reactive imidazolium-bridged dinucleotide intermediate in nonenzymatic RNA primer extension.

Walton T, Szostak JW
J Am Chem Soc. 2016;138(36):11996-2002 - PMID: 27552367 - DOI: 10.1021/jacs.6b07977

Nonenzymatic copying of RNA templates containing all four letters is catalyzed by activated oligonucleotides.

Prywes N, Blain JC, Del Frate F, Szostak JW
Elife. 2016;5:ePub - PMID: 27351102 - PMCID: PMC4959843 - DOI: 10.7554/eLife.17756

Oligoarginine peptides slow strand annealing and assist non-enzymatic RNA replication.

Jia TZ, Fahrenbach AC, Kamat NP, Adamala KP, Szostak JW
Nat Chem. 2016;8(10):915-21 - PMID: 27657866 - PMCID: PMC5061144 - DOI: 10.1038/nchem.2551

Thiolated uridine substrates and templates improve the rate and fidelity of ribozyme-catalyzed RNA copying.

Prywes N, Michaels YS, Pal A, Oh SS, Szostak JW
Chem Commun (Camb). 2016;52(39):6529-32 - PMID: 27109314 - DOI: 10.1039/c6cc02692c

Collaboration between primitive cell membranes and soluble catalysts.

Adamala KP, Engelhart AE, Szostak JW
Nat Commun. 2016;7:11041 - PMID: 26996603 - PMCID: PMC4802160 - DOI: 10.1038/ncomms11041

Experimental and computational evidence for a loose transition state in phosphoroimidazolide hydrolysis.

Li L, Lelyveld VS, Prywes N, Szostak JW
J Am Chem Soc. 2016;138(12):3986-3989 - PMID: 26974265 - DOI: 10.1021/jacs.6b00784

A simple physical mechanism enables homeostasis in primitive cells.

Engelhart AE, Adamala KP, Szostak JW
Nat Chem. 2016;8(5):448-53 - PMID: 27102678 - PMCID: PMC4929987 - DOI: 10.1038/nchem.2475

Synthesis of activated 3'-amino-3'-deoxy-2-thio-thymidine, a superior substrate for the nonenzymatic copying of nucleic acid templates.

Izgu EC, Oh SS, Szostak JW
Chem Commun (Camb). 2016;52(18):3684-6 - PMID: 26857159 - DOI: 10.1039/c5cc10317g

Pinpointing RNA-protein crosslinks with site-specific stable isotope labeled oligonucleotides.

Lelyveld VS, Bjorkbom A, Ransey E, Sliz P, Szostak JW
J Am Chem Soc. 2015;137(49):15378-15381 - PMID: 26583201 - PMCID: PMC4697197 - DOI: 10.1021/jacs.5b10596

Bidirectional direct sequencing of non-canonical RNA by two-dimensional analysis of mass chromatograms.

Bjorkbom A, Lelyveld VS, Zhang S, Zhang W, Tam CP, Blain JC, Szostak JW
J Am Chem Soc. 2015;137(45):14430-14438 - PMID: 26495937 - DOI: 10.1021/jacs.5b09438

Thermodynamic insights into 2-thiouridine-enhanced RNA hybridization.

Larsen AT, Fahrenbach AC, Sheng J, Pian J, Szostak JW
Nucleic Acids Res. 2015;43(16):7675-87 - PMID: 26240387 - PMCID: PMC4652770 - DOI: 10.1093/nar/gkv761

Electrostatic Localization of RNA to Protocell Membranes by Cationic Hydrophobic Peptides.

Kamat NP, Tobé S, Hill IT, Szostak JW
Angew Chem Int Ed Engl. 2015;54(40):11735-11739 - PMID: 26223820 - PMCID: PMC4600236 - DOI: 10.1002/anie.201505742

Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations.

Adamala K, Engelhart AE, Kamat NP, Jin L, Szostak JW
Nat Protoc. 2015;10(6):927-938 - PMID: 26020615 - PMCID: PMC4982460 - DOI: 10.1038/nprot.2015.054

Uncovering the Thermodynamics of Monomer Binding for RNA Replication.

Izgu EC, Fahrenbach AC, Zhang N, Li L, Zhang W, Larsen A, Blain JC, Szostak JW
J Am Chem Soc. 2015;137(19):6373-82 - PMID: 25901790 - PMCID: PMC4984997 - DOI: 10.1021/jacs.5b02707

Replacing Uridine with 2-Thio-Uridine Enhances the Rate and Fidelity of Nonenzymatic RNA Primer Extension.

Heuberger BD, Pal A, Del Frate F, Topkar VV, Szostak JW
J Am Chem Soc. 2015;137(7):2769-75 - PMID: 25654265 - PMCID: PMC4985000 - DOI: 10.1021/jacs.5b00445

Generation of functional RNAs from inactive oligonucleotide complexes by non-enzymatic primer extension.

Adamala K, Engelhart AE, Szostak JW
J Am Chem Soc. 2014;137(1):483-9 - PMID: 25521912 - PMCID: PMC4984999 - DOI: 10.1021/ja511564d

Controlled growth of filamentous fatty acid vesicles under flow.

Hentrich C, Szostak JW
Langmuir. 2014;30(49):14916-25 - PMID: 25402759 - PMCID: PMC4985002 - DOI: 10.1021/la503933x

Chain-Length Heterogeneity Allows for the Assembly of Fatty Acid Vesicles in Dilute Solutions.

Budin I, Prwyes N, Zhang N, Szostak JW
Biophys J. 2014;107(7):1582-1590 - PMID: 25296310 - PMCID: PMC4190651 - DOI: 10.1016/j.bpj.2014.07.067

Crystal Structure Studies of RNA Duplexes Containing s2U:A and s2U:U Base Pairs.

Sheng J, Larsen A, Heuberger BD, Blain JC, Szostak JW
J Am Chem Soc. 2014;136(39):13916-24 - PMID: 25188906 - PMCID: PMC4183603 - DOI: 10.1021/ja508015a

Progress Toward Synthetic Cells.

Blain JC, Szostak JW
Annu Rev Biochem. 2014;83:615-640 - PMID: 24606140 - DOI: 10.1146/annurev-biochem-080411-124036

Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets.

Jia TZ, Hentrich C, Szostak JW
Orig Life Evol Biosph. 2014;44(1):1-12 - PMID: 24577897 - PMCID: PMC4141154 - DOI: 10.1007/s11084-014-9355-8

Structural insights into the effects of 2'-5' linkages on the RNA duplex.

Sheng J, Li L, Engelhart AE, Gan J, Wang J, Szostak JW
Proc Natl Acad Sci U S A. 2014;111(8):3050-5 - PMID: 24516151 - PMCID: PMC3939906 - DOI: 10.1073/pnas.1317799111

The Free Energy Landscape of Pseudorotation in 3'-5' and 2'-5' Linked Nucleic Acids.

Li L, Szostak JW
J Am Chem Soc. 2014;136(7):2858-65 - PMID: 24499340 - PMCID: PMC3982932 - DOI: 10.1021/ja412079b

Synthesis and Non-Enzymatic Template-Directed Polymerization of 2'-Amino-2'-Deoxythreose Nucleotides.

Blain JC, Ricardo A, Szostak JW
J Am Chem Soc. 2014;136(5):2033-9 - PMID: 24409991 - PMCID: PMC4105081 - DOI: 10.1021/ja411950n

Preparation of Fatty Acid micelles.

Zhu TF, Budin I, Szostak JW
Methods Enzymol. 2013;533:283-8 - PMID: 24182934 - PMCID: PMC4310231 - DOI: 10.1016/B978-0-12-420067-8.00022-2

Vesicle Extrusion Through Polycarbonate Track-etched Membranes using a Hand-held Mini-extruder.

Zhu TF, Budin I, Szostak JW
Methods Enzymol. 2013;533:275-82 - PMID: 24182933 - PMCID: PMC4310236 - DOI: 10.1016/B978-0-12-420067-8.00021-0

Preparation of Fatty Acid or Phospholipid Vesicles by Thin-film Rehydration.

Zhu TF, Budin I, Szostak JW
Methods Enzymol. 2013;533:267-74 - PMID: 24182932 - PMCID: PMC4310237 - DOI: 10.1016/B978-0-12-420067-8.00020-9

Nonenzymatic template-directed RNA synthesis inside model protocells.

Adamala K, Szostak JW
Science. 2013;342(6162):1098-100 - PMID: 24288333 - PMCID: PMC4104020 - DOI: 10.1126/science.1241888

mRNA display: from basic principles to macrocycle drug discovery.

Josephson K, Ricardo A, Szostak JW
Drug Discov Today. 2013;19(4):388-399 - PMID: 24157402 - DOI: 10.1016/j.drudis.2013.10.011

Fast and accurate nonenzymatic copying of an RNA-like synthetic genetic polymer.

Zhang S, Blain JC, Zielinska D, Gryaznov SM, Szostak JW
Proc Natl Acad Sci U S A. 2013;110(44):17732-7 - PMID: 24101473 - PMCID: PMC3816440 - DOI: 10.1073/pnas.1312329110

Competition between model protocells driven by an encapsulated catalyst.

Adamala K, Szostak JW
Nat Chem. 2013;5(7):634 - PMID: 23787756

Competition between model protocells driven by an encapsulated catalyst.

Adamala K, Szostak JW
Nat Chem. 2013;5(6):495-501 - PMID: 23695631 - PMCID: PMC4041014 - DOI: 10.1038/nchem.1650

Functional RNAs exhibit tolerance for non-heritable 2'-5' versus 3'-5' backbone heterogeneity.

Engelhart AE, Powner MW, Szostak JW
Nat Chem. 2013;5(5):390-4 - PMID: 23609089 - PMCID: PMC4088963 - DOI: 10.1038/nchem.1623

Synthesis of N3'-P5'-linked phosphoramidate DNA by nonenzymatic template-directed primer extension.

Zhang S, Zhang N, Blain JC, Szostak JW
J Am Chem Soc. 2013;135(2):924-32 - PMID: 23252395 - PMCID: PMC3548433 - DOI: 10.1021/ja311164j

Synthesis of N3´-P5´-Phosphoramidate DNA by Non-enzymatic Template-directed Primer Extension.

Zhang S, Zhang N, Blain JC, Szostak JW
J Am Chem Soc. 2012;135(2):924-32 - PMID: 23252395 - PMCID: PMC3548433 - DOI: 10.1021/ja311164j

Concentration-driven growth of model protocell membranes.

Budin I, Debnath A, Szostak JW
J Am Chem Soc. 2012;134(51):20812-9 - PMID: 23198690 - PMCID: PMC3530389 - DOI: 10.1021/ja310382d

Concentration-driven growth of model protocell membranes.

Budin I, Debnath A, Szostak JW
J Am Chem Soc. 2012;134(51):20812-9 - PMID: 23198690 - PMCID: PMC3530389 - DOI: 10.1021/ja310382d

Multicomponent assembly of proposed DNA precursors in water.

Powner MW, Zheng SL, Szostak JW
J Am Chem Soc. 2012;134(33):13889-95 - PMID: 22839703 - PMCID: PMC3424849 - DOI: 10.1021/ja306176n

Multicomponent assembly of proposed DNA precursors in water.

Powner MW, Zheng SL, Szostak JW
J Am Chem Soc. 2012;134(33):13889-95 - PMID: 22839703 - PMCID: PMC3424849 - DOI: 10.1021/ja306176n

Photochemically driven redox chemistry induces protocell membrane pearling and division.

Zhu TF, Adamala K, Zhang N, Szostak JW
Proc Natl Acad Sci U S A. 2012;109(25):9828-32 - PMID: 22665773 - PMCID: PMC3382484 - DOI: 10.1073/pnas.1203212109

In vitro selection of functional lantipeptides.

Hofmann FT, Szostak JW, Seebeck FP
J Am Chem Soc. 2012;134(19):8038-41 - PMID: 22545861 - PMCID: PMC3353655 - DOI: 10.1021/ja302082d

In Vitro Selection of Highly Modified Cyclic Peptides That Act as Tight Binding Inhibitors.

Guillen Schlippe YV, Hartman MC, Josephson K, Szostak JW
J Am Chem Soc. 2012;134(25):10469-77 - PMID: 22428867 - PMCID: PMC3384292 - DOI: 10.1021/ja301017y

Activated ribonucleotides undergo a sugar pucker switch upon binding to a single-stranded RNA template.

Zhang N, Zhang S, Szostak JW
J Am Chem Soc. 2012;134(8):3691-4 - PMID: 22296305 - PMCID: PMC3448298 - DOI: 10.1021/ja212027q

Attempts to define life do not help to understand the origin of life.

Szostak JW
J Biomol Struct Dyn. 2012;29(4):599-600 - PMID: 22208251 - PMCID: PMC4208307 - DOI: 10.1080/073911012010524998

An optimal degree of physical and chemical heterogeneity for the origin of life?

Szostak JW
Philos Trans R Soc Lond B Biol Sci. 2011;366(1580):2894-901 - PMID: 21930580 - PMCID: PMC3158921 - DOI: 10.1098/rstb.2011.0140

Evolution of functional nucleic acids in the presence of nonheritable backbone heterogeneity.

Trevino SG, Zhang N, Elenko MP, Lupták A, Szostak JW
Proc Natl Acad Sci U S A. 2011;108(33):13492-7 - PMID: 21825162 - PMCID: PMC3158193 - DOI: 10.1073/pnas.1107113108

Artificial lantipeptides from in vitro translations.

Seebeck FP, Ricardo A, Szostak JW
Chem Commun (Camb). 2011;47(21):6141-3 - PMID: 21528125 - DOI: 10.1039/c0cc05663d

Physical effects underlying the transition from primitive to modern cell membranes.

Budin I, Szostak JW
Proc Natl Acad Sci U S A. 2011;108(13):5249-54 - PMID: 21402937 - PMCID: PMC3069173 - DOI: 10.1073/pnas.1100498108

Optimal codon choice can improve the efficiency and fidelity of N-methyl amino acid incorporation into peptides by in-vitro translation.

Subtelny AO, Hartman MC, Szostak JW
Angew Chem Int Ed Engl. 2011;50(14):3164-7 - PMID: 21381166 - PMCID: PMC4006073 - DOI: 10.1002/anie.201007686

Chemoselective multicomponent one-pot assembly of purine precursors in water.

Powner MW, Sutherland JD, Szostak JW
J Am Chem Soc. 2010;132(46):16677-88 - PMID: 21043502 - PMCID: PMC2984373 - DOI: 10.1021/ja108197s

DNA ends: just the beginning (Nobel lecture).

Szostak JW
Angew Chem Int Ed Engl. 2010;49(41):7386-404 - PMID: 20818632 - DOI: 10.1002/anie.201000635

Single-molecule binding experiments on long time scales.

Elenko MP, Szostak JW, van Oijen AM
Rev Sci Instrum. 2010;81(8):083705 - PMID: 20815611 - DOI: 10.1063/1.3473936

Expanding roles for diverse physical phenomena during the origin of life.

Budin I, Szostak JW
Annu Rev Biophys. 2010;39:245-63 - PMID: 20192779 - PMCID: PMC4992673 - DOI: 10.1146/annurev.biophys.050708.133753

The origins of cellular life.

Schrum JP, Zhu TF, Szostak JW
Cold Spring Harb Perspect Biol. 2010;2(9):a002212 - PMID: 20484387 - PMCID: PMC2926753 - DOI: 10.1101/cshperspect.a002212

Effect of stalling after mismatches on the error catastrophe in nonenzymatic nucleic acid replication.

Rajamani S, Ichida JK, Antal T, Treco DA, Leu K, Nowak MA, Szostak JW, Chen IA
J Am Chem Soc. 2010;132(16):5880-5 - PMID: 20359213 - PMCID: PMC2857888 - DOI: 10.1021/ja100780p

Flip-flop-induced relaxation of bending energy: implications for membrane remodeling.

Bruckner RJ, Mansy SS, Ricardo A, Mahadevan L, Szostak JW
Biophys J. 2009;97(12):3113-22 - PMID: 20006948 - PMCID: PMC2793357 - DOI: 10.1016/j.bpj.2009.09.025

Efficient and rapid template-directed nucleic acid copying using 2'-amino-2',3'-dideoxyribonucleoside-5'-phosphorimidazolide monomers.

Schrum JP, Ricardo A, Krishnamurthy M, Blain JC, Szostak JW
J Am Chem Soc. 2009;131(40):14560-70 - PMID: 19757789 - PMCID: PMC2759813 - DOI: 10.1021/ja906557v

Reconstructing the emergence of cellular life through the synthesis of model protocells.

Mansy SS, Szostak JW
Cold Spring Harb Symp Quant Biol. 2009;74:47-54 - PMID: 19734203 - DOI: 10.1101/sqb.2009.74.014

Origin of life on earth.

Ricardo A, Szostak JW
Sci Am. 2009;301(3):54-61 - PMID: 19708528

Conformational analysis of DNA repair intermediates by time-resolved fluorescence spectroscopy.

Lin S, Horning DP, Szostak JW, Chaput JC
J Phys Chem A. 2009;113(35):9585-7 - PMID: 19673467 - PMCID: PMC2733762 - DOI: 10.1021/jp906746w

Formation of protocell-like vesicles in a thermal diffusion column.

Budin I, Bruckner RJ, Szostak JW
J Am Chem Soc. 2009;131(28):9628-9 - PMID: 19601679 - PMCID: PMC2710859 - DOI: 10.1021/ja9029818

Single-molecule imaging of an in vitro-evolved RNA aptamer reveals homogeneous ligand binding kinetics.

Elenko MP, Szostak JW, van Oijen AM
J Am Chem Soc. 2009;131(29):9866-7 - PMID: 19572753 - PMCID: PMC2716146 - DOI: 10.1021/ja901880v

Origins of life: Systems chemistry on early Earth.

Szostak JW
Nature. 2009;459(7244):171-2 - PMID: 19444196 - DOI: 10.1038/459171a

Retraction for Litovchick and Szostak. "Selection of cyclic peptide aptamers to HCV IRES RNA using mRNA display".

Litovchick A, Szostak JW
Proc Natl Acad Sci U S A. 2009;106(17):7263 - PMID: 19380733 - PMCID: PMC2678432 - DOI: 10.1073/pnas.0903110106

Preparation of large monodisperse vesicles.

Zhu TF, Szostak JW
PLoS One. 2009;4(4):e5009 - PMID: 19347043 - PMCID: PMC2661144 - DOI: 10.1371/journal.pone.0005009

Coupled growth and division of model protocell membranes.

Zhu TF, Szostak JW
J Am Chem Soc. 2009;131(15):5705-13 - PMID: 19323552 - PMCID: PMC2669828 - DOI: 10.1021/ja900919c

Enzymatic primer-extension with glycerol-nucleoside triphosphates on DNA templates.

Chen JJ, Tsai CH, Cai X, Horhota AT, McLaughlin LW, Szostak JW
PLoS One. 2009;4(3):e4949 - PMID: 19305495 - PMCID: PMC2654545 - DOI: 10.1371/journal.pone.0004949

N2'-->p3' phosphoramidate glycerol nucleic acid as a potential alternative genetic system.

Chen JJ, Cai X, Szostak JW
J Am Chem Soc. 2009;131(6):2119-21 - PMID: 19166350 - PMCID: PMC2722806 - DOI: 10.1021/ja809069b

Selection of cyclic peptide aptamers to HCV IRES RNA using mRNA display.

Litovchick A, Szostak JW
Proc Natl Acad Sci U S A. 2008;105(40):15293-8 - PMID: 18824687 - PMCID: PMC2556359 - DOI: 10.1073/pnas.0805837105

Thermostability of model protocell membranes.

Mansy SS, Szostak JW
Proc Natl Acad Sci U S A. 2008;105(36):13351-5 - PMID: 18768808 - PMCID: PMC2528865 - DOI: 10.1073/pnas.0805086105

Template-directed synthesis of a genetic polymer in a model protocell.

Mansy SS, Schrum JP, Krishnamurthy M, Tobé S, Treco DA, Szostak JW
Nature. 2008;454(7200):122-5 - PMID: 18528332 - PMCID: PMC2743009 - DOI: 10.1038/nature07018

Ribosomal synthesis of N-methyl peptides.

Subtelny AO, Hartman MC, Szostak JW
J Am Chem Soc. 2008;130(19):6131-6 - PMID: 18402453 - PMCID: PMC2728122 - DOI: 10.1021/ja710016v

Metal-ion catalyzed polymerization in the eutectic phase in water-ice: a possible approach to template-directed RNA polymerization.

Monnard PA, Szostak JW
J Inorg Biochem. 2008;102(5-6):1104-11 - PMID: 18329104 - DOI: 10.1016/j.jinorgbio.2008.01.026

An expanded set of amino acid analogs for the ribosomal translation of unnatural peptides.

Hartman MC, Josephson K, Lin CW, Szostak JW
PLoS One. 2007;2(10):e972 - PMID: 17912351 - PMCID: PMC1989143 - DOI: 10.1371/journal.pone.0000972

Enzymatic synthesis of DNA on glycerol nucleic acid templates without stable duplex formation between product and template.

Tsai CH, Chen J, Szostak JW
Proc Natl Acad Sci U S A. 2007;104(37):14598-603 - PMID: 17785419 - PMCID: PMC1976233 - DOI: 10.1073/pnas.0704211104

Selection and evolution of enzymes from a partially randomized non-catalytic scaffold.

Seelig B, Szostak JW
Nature. 2007;448(7155):828-31 - PMID: 17700701 - PMCID: PMC4476047 - DOI: 10.1038/nature06032

Structure and evolutionary analysis of a non-biological ATP-binding protein.

Mansy SS, Zhang J, Kümmerle R, Nilsson M, Chou JJ, Szostak JW, Chaput JC
J Mol Biol. 2007;371(2):501-13 - PMID: 17583732 - DOI: 10.1016/j.jmb.2007.05.062

Structural insights into the evolution of a non-biological protein: importance of surface residues in protein fold optimization.

Smith MD, Rosenow MA, Wang M, Allen JP, Szostak JW, Chaput JC
PLoS One. 2007;2(5):e467 - PMID: 17520026 - PMCID: PMC1867856 - DOI: 10.1371/journal.pone.0000467

Functional information and the emergence of biocomplexity.

Hazen RM, Griffin PL, Carothers JM, Szostak JW
Proc Natl Acad Sci U S A. 2007;104 Suppl 1:8574-81 - PMID: 17494745 - PMCID: PMC1876432 - DOI: 10.1073/pnas.0701744104

2',3'-Dideoxy-3'-thionucleoside triphosphates: syntheses and polymerase substrate activities.

Meena , Sam M, Pierce K, Szostak JW, McLaughlin LW
Org Lett. 2007;9(6):1161-3 - PMID: 17319676 - DOI: 10.1021/ol070147w

Glycerol nucleoside triphosphates: synthesis and polymerase substrate activities.

Horhota AT, Szostak JW, McLaughlin LW
Org Lett. 2006;8(23):5345-7 - PMID: 17078714 - DOI: 10.1021/ol062232u

Telomeres and telomerase: the path from maize, Tetrahymena and yeast to human cancer and aging.

Blackburn EH, Greider CW, Szostak JW
Nat Med. 2006;12(10):1133-8 - PMID: 17024208 - DOI: 10.1038/nm1006-1133

A genomewide search for ribozymes reveals an HDV-like sequence in the human CPEB3 gene.

Salehi-Ashtiani K, Lupták A, Litovchick A, Szostak JW
Science. 2006;313(5794):1788-92 - PMID: 16990549 - DOI: 10.1126/science.1129308

Mineral surface directed membrane assembly.

Hanczyc MM, Mansy SS, Szostak JW
Orig Life Evol Biosph. 2006;37(1):67-82 - PMID: 16909329 - DOI: 10.1007/s11084-006-9018-5

Aptamers selected for higher-affinity binding are not more specific for the target ligand.

Carothers JM, Oestreich SC, Szostak JW
J Am Chem Soc. 2006;128(24):7929-37 - PMID: 16771507 - PMCID: PMC4287982 - DOI: 10.1021/ja060952q

Ribosomal synthesis of dehydroalanine-containing peptides.

Seebeck FP, Szostak JW
J Am Chem Soc. 2006;128(22):7150-1 - PMID: 16734454 - PMCID: PMC2572134 - DOI: 10.1021/ja060966w

Enzymatic aminoacylation of tRNA with unnatural amino acids.

Hartman MC, Josephson K, Szostak JW
Proc Natl Acad Sci U S A. 2006;103(12):4356-61 - PMID: 16537388 - PMCID: PMC1450175 - DOI: 10.1073/pnas.0509219103

Solution structure of an informationally complex high-affinity RNA aptamer to GTP.

Carothers JM, Davis JH, Chou JJ, Szostak JW
RNA. 2006;12(4):567-79 - PMID: 16510427 - PMCID: PMC1421093 - DOI: 10.1261/rna.2251306

Directed evolution of ATP binding proteins from a zinc finger domain by using mRNA display.

Cho GS, Szostak JW
Chem Biol. 2006;13(2):139-47 - PMID: 16492562 - DOI: 10.1016/j.chembiol.2005.10.015

Shrink-wrap vesicles.

Fujikawa SM, Chen IA, Szostak JW
Langmuir. 2005;21(26):12124-9 - PMID: 16342983 - PMCID: PMC2548281 - DOI: 10.1021/la052590q

In vitro selection of RNA aptamers against a composite small molecule-protein surface.

Plummer KA, Carothers JM, Yoshimura M, Szostak JW, Verdine GL
Nucleic Acids Res. 2005;33(17):5602-10 - PMID: 16199752 - PMCID: PMC1240114 - DOI: 10.1093/nar/gki867

High fidelity TNA synthesis by Therminator polymerase.

Ichida JK, Horhota A, Zou K, McLaughlin LW, Szostak JW
Nucleic Acids Res. 2005;33(16):5219-25 - PMID: 16157867 - PMCID: PMC1214552 - DOI: 10.1093/nar/gki840

RNA catalysis in model protocell vesicles.

Chen IA, Salehi-Ashtiani K, Szostak JW
J Am Chem Soc. 2005;127(38):13213-9 - PMID: 16173749 - PMCID: PMC5072289 - DOI: 10.1021/ja051784p

Ribosomal synthesis of unnatural peptides.

Josephson K, Hartman MC, Szostak JW
J Am Chem Soc. 2005;127(33):11727-35 - PMID: 16104750 - DOI: 10.1021/ja0515809

Kinetic analysis of an efficient DNA-dependent TNA polymerase.

Horhota A, Zou K, Ichida JK, Yu B, McLaughlin LW, Szostak JW, Chaput JC
J Am Chem Soc. 2005;127(20):7427-34 - PMID: 15898792 - PMCID: PMC5042361 - DOI: 10.1021/ja0428255

Scanning the human proteome for calmodulin-binding proteins.

Shen X, Valencia CA, Szostak JW, Szostak J, Dong B, Liu R
Proc Natl Acad Sci U S A. 2005;102(17):5969-74 - PMID: 15840729 - PMCID: PMC1087907 - DOI: 10.1073/pnas.0407928102

Semipermeable lipid bilayers exhibit diastereoselectivity favoring ribose.

Sacerdote MG, Szostak JW
Proc Natl Acad Sci U S A. 2005;102(17):6004-8 - PMID: 15831588 - PMCID: PMC556129 - DOI: 10.1073/pnas.0408440102

Synthesis of alpha-L-threofuranosyl nucleoside triphosphates (tNTPs).

Zou K, Horhota A, Yu B, Szostak JW, McLaughlin LW
Org Lett. 2005;7(8):1485-7 - PMID: 15816733 - PMCID: PMC5022784 - DOI: 10.1021/ol050081+

An in vitro selection system for TNA.

Ichida JK, Zou K, Horhota A, Yu B, McLaughlin LW, Szostak JW
J Am Chem Soc. 2005;127(9):2802-3 - PMID: 15740086 - PMCID: PMC5072288 - DOI: 10.1021/ja045364w

Replicating vesicles as models of primitive cell growth and division.

Hanczyc MM, Szostak JW
Curr Opin Chem Biol. 2004;8(6):660-4 - PMID: 15556412 - DOI: 10.1016/j.cbpa.2004.10.002

The emergence of competition between model protocells.

Chen IA, Roberts RW, Szostak JW
Science. 2004;305(5689):1474-6 - PMID: 15353806 - PMCID: PMC4484590 - DOI: 10.1126/science.1100757

A kinetic study of the growth of fatty acid vesicles.

Chen IA, Szostak JW
Biophys J. 2004;87(2):988-98 - PMID: 15298905 - PMCID: PMC1304506 - DOI: 10.1529/biophysj.104.039875

A small aptamer with strong and specific recognition of the triphosphate of ATP.

Sazani PL, Larralde R, Szostak JW
J Am Chem Soc. 2004;126(27):8370-1 - PMID: 15237981 - PMCID: PMC4983724 - DOI: 10.1021/ja049171k

Evolutionary optimization of a nonbiological ATP binding protein for improved folding stability.

Chaput JC, Szostak JW
Chem Biol. 2004;11(6):865-74 - PMID: 15217619 - DOI: 10.1016/j.chembiol.2004.04.006

Membrane growth can generate a transmembrane pH gradient in fatty acid vesicles.

Chen IA, Szostak JW
Proc Natl Acad Sci U S A. 2004;101(21):7965-70 - PMID: 15148394 - PMCID: PMC419540 - DOI: 10.1073/pnas.0308045101

Informational complexity and functional activity of RNA structures.

Carothers JM, Oestreich SC, Davis JH, Szostak JW
J Am Chem Soc. 2004;126(16):5130-7 - PMID: 15099096 - PMCID: PMC5042360 - DOI: 10.1021/ja031504a

Evolution of aptamers with a new specificity and new secondary structures from an ATP aptamer.

Huang Z, Szostak JW
RNA. 2003;9(12):1456-63 - PMID: 14624002 - PMCID: PMC1370500

Experimental models of primitive cellular compartments: encapsulation, growth, and division.

Hanczyc MM, Fujikawa SM, Szostak JW
Science. 2003;302(5645):618-22 - PMID: 14576428 - PMCID: PMC4484575 - DOI: 10.1126/science.1089904

TNA synthesis by DNA polymerases.

Chaput JC, Szostak JW
J Am Chem Soc. 2003;125(31):9274-5 - PMID: 12889939 - DOI: 10.1021/ja035917n

A novel, modification-dependent ATP-binding aptamer selected from an RNA library incorporating a cationic functionality.

Vaish NK, Larralde R, Fraley AW, Szostak JW, McLaughlin LW
Biochemistry. 2003;42(29):8842-51 - PMID: 12873145 - DOI: 10.1021/bi027354i

Functional information: Molecular messages.

Szostak JW
Nature. 2003;423(6941):689 - PMID: 12802312 - DOI: 10.1038/423689a

Selective labeling and detection of specific RNAs in an RNA mixture.

Huang Z, Szostak JW
Anal Biochem. 2003;315(1):129-33 - PMID: 12672421

DNA polymerase-mediated DNA synthesis on a TNA template.

Chaput JC, Ichida JK, Szostak JW
J Am Chem Soc. 2003;125(4):856-7 - PMID: 12537469 - DOI: 10.1021/ja028589k

Molecular biology: RNA gets a grip on translation.

Szostak JW
Nature. 2002;419(6910):890-1 - PMID: 12410294 - DOI: 10.1038/419890a

Identification of epitope-like consensus motifs using mRNA display.

Baggio R, Burgstaller P, Hale SP, Putney AR, Lane M, Lipovsek D, Wright MC, Roberts RW, Liu R, Szostak JW, Wagner RW
J Mol Recognit. 2002;15(3):126-34 - PMID: 12203838 - DOI: 10.1002/jmr.567

Isolation of high-affinity GTP aptamers from partially structured RNA libraries.

Davis JH, Szostak JW
Proc Natl Acad Sci U S A. 2002;99(18):11616-21 - PMID: 12185247 - PMCID: PMC129318 - DOI: 10.1073/pnas.182095699

One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag.

Keefe AD, Wilson DS, Seelig B, Szostak JW
Protein Expr Purif. 2001;23(3):440-6 - PMID: 11722181 - DOI: 10.1006/prep.2001.1515

In vitro evolution suggests multiple origins for the hammerhead ribozyme.

Salehi-Ashtiani K, Szostak JW
Nature. 2001;414(6859):82-4 - PMID: 11689947 - DOI: 10.1038/35102081

Functional proteins from a random-sequence library.

Keefe AD, Szostak JW
Nature. 2001;410(6829):715-8 - PMID: 11287961 - PMCID: PMC4476321 - DOI: 10.1038/35070613

The use of mRNA display to select high-affinity protein-binding peptides.

Wilson DS, Keefe AD, Szostak JW
Proc Natl Acad Sci U S A. 2001;98(7):3750-5 - PMID: 11274392 - PMCID: PMC31124 - DOI: 10.1073/pnas.061028198

Synthesizing life.

Szostak JW, Bartel DP, Luisi PL
Nature. 2001;409(6818):387-90 - PMID: 11201752 - DOI: 10.1038/35053176

Optimized synthesis of RNA-protein fusions for in vitro protein selection.

Liu R, Barrick JE, Szostak JW, Roberts RW
Methods Enzymol. 2000;318:268-93 - PMID: 10889994

In vitro selection and directed evolution.

Szostak JW
Harvey Lect. 2000;93:95-118 - PMID: 10941421

Expanding the structural and functional diversity of RNA: analog uridine triphosphates as candidates for in vitro selection of nucleic acids.

Vaish NK, Fraley AW, Szostak JW, McLaughlin LW
Nucleic Acids Res. 2000;28(17):3316-22 - PMID: 10954600 - PMCID: PMC110695

In vitro selection of functional nucleic acids.

Wilson DS, Szostak JW
Annu Rev Biochem. 2000;68:611-47 - PMID: 10872462 - DOI: 10.1146/annurev.biochem.68.1.611

Constructing high complexity synthetic libraries of long ORFs using in vitro selection.

Cho G, Keefe AD, Liu R, Wilson DS, Szostak JW
J Mol Biol. 2000;297(2):309-19 - PMID: 10715203 - DOI: 10.1006/jmbi.2000.3571

Ribozyme-catalyzed tRNA aminoacylation.

Lee N, Bessho Y, Wei K, Szostak JW, Suga H
Nat Struct Biol. 2000;7(1):28-33 - PMID: 10625423 - DOI: 10.1038/71225

Isolation and characterization of fluorophore-binding RNA aptamers.

Holeman LA, Robinson SL, Szostak JW, Wilson C
Fold Des. 1999;3(6):423-31 - PMID: 9889155 - DOI: 10.1016/S1359-0278(98)00059-5

Isolation of a fluorophore-specific DNA aptamer with weak redox activity.

Wilson C, Szostak JW
Chem Biol. 1998;5(11):609-17 - PMID: 9831529

Unusual metal ion catalysis in an acyl-transferase ribozyme.

Suga H, Cowan JA, Szostak JW
Biochemistry. 1998;37(28):10118-25 - PMID: 9665717 - DOI: 10.1021/bi980432a

Structural and kinetic characterization of an acyl transferase ribozyme.

Suga H, Lohse PA, Szostak JW
J Am Chem Soc. 1998;120(6):1151-6 - PMID: 11541113

RNA-peptide fusions for the in vitro selection of peptides and proteins.

Roberts RW, Szostak JW
Proc Natl Acad Sci U S A. 1997;94(23):12297-302 - PMID: 9356443 - PMCID: PMC24913

Mutant ATP-binding RNA aptamers reveal the structural basis for ligand binding.

Dieckmann T, Butcher SE, Sassanfar M, Szostak JW, Feigon J
J Mol Biol. 1997;273(2):467-78 - PMID: 9344753 - DOI: 10.1006/jmbi.1997.1329

In vitro selection of catalytic RNAs.

Chapman KB, Szostak JW
Curr Opin Struct Biol. 1997;4:618-22 - PMID: 11539575

Isolation of novel ribozymes that ligate AMP-activated RNA substrates.

Hager AJ, Szostak JW
Chem Biol. 1997;4(8):607-17 - PMID: 9281527

Introduction: Combinatorial Chemistry.

Szostak JW
Chem Rev. 1997;97(2):347-348 - PMID: 11848873

A simple method for 3'-labeling of RNA.

Huang Z, Szostak JW
Nucleic Acids Res. 1996;24(21):4360-1 - PMID: 8932396 - PMCID: PMC146232

Ribozymes: aiming at RNA replication and protein synthesis.

Hager AJ, Pollard JD, Szostak JW
Chem Biol. 1996;3(9):717-25 - PMID: 8939686

Ribozyme-catalysed amino-acid transfer reactions.

Lohse PA, Szostak JW
Nature. 1996;381(6581):442-4 - PMID: 8632803 - DOI: 10.1038/381442a0

Nonenzymatic, template-directed ligation of oligoribonucleotides is highly regioselective for the formation of 3'-5' phosphodiester bonds.

Rohatgi R, Bartel DP, Szostak JW
J Am Chem Soc. 1996;118(14):3340-4 - PMID: 11539268

Kinetic and mechanistic analysis of nonenzymatic, template-directed oligoribonucleotide ligation.

Rohatgi R, Bartel DP, Szostak JW
J Am Chem Soc. 1996;118(14):3332-9 - PMID: 11539267

Chance and necessity in the selection of nucleic acid catalysts.

Lorsch JR, Szostak JW
Acc Chem Res. 1996;29(2):103-10 - PMID: 11539421

Kinetic and thermodynamic characterization of the reaction catalyzed by a polynucleotide kinase ribozyme.

Lorsch JR, Szostak JW
Biochemistry. 1995;34(46):15315-27 - PMID: 7578148

Reverse transcriptase reads through a 2'-5'linkage and a 2'-thiophosphate in a template.

Lorsch JR, Bartel DP, Szostak JW
Nucleic Acids Res. 1995;23(15):2811-4 - PMID: 7544885 - PMCID: PMC307115

Structurally complex and highly active RNA ligases derived from random RNA sequences.

Ekland EH, Szostak JW, Bartel DP
Science. 1995;269(5222):364-70 - PMID: 7618102

A DNA metalloenzyme with DNA ligase activity.

Cuenoud B, Szostak JW
Nature. 1995;375(6532):611-4 - PMID: 7791880 - DOI: 10.1038/375611a0

Isolation of a ribozyme with 5'-5' ligase activity.

Chapman KB, Szostak JW
Chem Biol. 1995;2(5):325-33 - PMID: 9383434

In vitro evolution of a self-alkylating ribozyme.

Wilson C, Szostak JW
Nature. 1995;374(6525):777-82 - PMID: 7723823 - DOI: 10.1038/374777a0

Total chemical synthesis of a ribozyme derived from a group I intron.

Whoriskey SK, Usman N, Szostak JW
Proc Natl Acad Sci U S A. 1995;92(7):2465-9 - PMID: 7708666 - PMCID: PMC42238

RNA aptamers that bind flavin and nicotinamide redox cofactors.

Lauhon CT, Szostak JW
J Am Chem Soc. 1995;117(4):1246-57 - PMID: 11539282

A DNA aptamer that binds adenosine and ATP.

Huizenga DE, Szostak JW
Biochemistry. 1995;34(2):656-65 - PMID: 7819261

Suppression of mutations in the core of the Tetrahymena ribozyme by spermidine, ethanol and by substrate stabilization.

Hanna M, Szostak JW
Nucleic Acids Res. 1994;22(24):5326-31 - PMID: 7816622 - PMCID: PMC332078

In vitro evolution of new ribozymes with polynucleotide kinase activity.

Lorsch JR, Szostak JW
Nature. 1994;371(6492):31-6 - PMID: 7521014 - DOI: 10.1038/371031a0

1H NMR studies of the high-affinity Rev binding site of the Rev responsive element of HIV-1 mRNA: base pairing in the core binding element.

Peterson RD, Bartel DP, Szostak JW, Horvath SJ, Feigon J
Biochemistry. 1994;33(18):5357-66 - PMID: 8180157

Evidence that the guanosine substrate of the Tetrahymena ribozyme is bound in the anti conformation and that N7 contributes to binding.

Lin CW, Hanna M, Szostak JW
Biochemistry. 1994;33(9):2703-7 - PMID: 8117735

In vitro selection of RNA aptamers specific for cyanocobalamin.

Lorsch JR, Szostak JW
Biochemistry. 1994;33(4):973-82 - PMID: 7508262

In vitro genetic analysis of the hinge region between helical elements P5-P4-P6 and P7-P3-P8 in the sunY group I self-splicing intron.

Green R, Szostak JW
J Mol Biol. 1994;235(1):140-55 - PMID: 7507168

Isolation of new ribozymes from a large pool of random sequences [see comment].

Bartel DP, Szostak JW
Science. 1993;261(5127):1411-8 - PMID: 7690155

An RNA motif that binds ATP.

Sassanfar M, Szostak JW
Nature. 1993;364(6437):550-3 - PMID: 7687750 - DOI: 10.1038/364550a0

Ribozyme-catalyzed primer extension by trinucleotides: a model for the RNA-catalyzed replication of RNA.

Doudna JA, Usman N, Szostak JW
Biochemistry. 1993;32(8):2111-5 - PMID: 7680575

Ribozymes. Evolution ex vivo.

Szostak JW
Nature. 1993;361(6408):119-20 - PMID: 7678448 - DOI: 10.1038/361119a0

Selection of a ribozyme that functions as a superior template in a self-copying reaction.

Green R, Szostak JW
Science. 1992;258(5090):1910-5 - PMID: 1470913

Meiotic recombination on artificial chromosomes in yeast.

Ross LO, Treco D, Nicolas A, Szostak JW, Dawson D
Genetics. 1992;131(3):541-50 - PMID: 1385793 - PMCID: PMC1205028

ARD1 and NAT1 proteins form a complex that has N-terminal acetyltransferase activity.

Park EC, Szostak JW
EMBO J. 1992;11(6):2087-93 - PMID: 1600941 - PMCID: PMC556675

In vitro genetics.

Szostak JW
Trends Biochem Sci. 1992;17(3):89-93 - PMID: 1384177

Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures.

Ellington AD, Szostak JW
Nature. 1992;355(6363):850-2 - PMID: 1538766 - DOI: 10.1038/355850a0

A strategy for the generation of conditional mutations by protein destabilization.

Park EC, Finley D, Szostak JW
Proc Natl Acad Sci U S A. 1992;89(4):1249-52 - PMID: 1311090 - PMCID: PMC48426

Genetic and physical analyses of sister chromatid exchange in yeast meiosis.

Sun H, Dawson D, Szostak JW
Mol Cell Biol. 1991;11(12):6328-36 - PMID: 1944292 - PMCID: PMC361828

HIV-1 Rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA.

Bartel DP, Zapp ML, Green MR, Szostak JW
Cell. 1991;67(3):529-36 - PMID: 1934059

Synthesis of RNA containing inosine: analysis of the sequence requirements for the 5' splice site of the Tetrahymena group I intron.

Green R, Szostak JW, Benner SA, Rich A, Usman N
Nucleic Acids Res. 1991;19(15):4161-6 - PMID: 1714564 - PMCID: PMC328556

Template-directed primer extension catalyzed by the Tetrahymena ribozyme.

Bartel DP, Doudna JA, Usman N, Szostak JW
Mol Cell Biol. 1991;11(6):3390-4 - PMID: 2038341 - PMCID: PMC360195

Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site.

Sun H, Treco D, Szostak JW
Cell. 1991;64(6):1155-61 - PMID: 2004421

Mutations in a semiconserved region of the Tetrahymena intron.

Pace U, Szostak JW
FEBS Lett. 1991;280(1):171-4 - PMID: 2009960

A multisubunit ribozyme that is a catalyst of and template for complementary strand RNA synthesis.

Doudna JA, Couture S, Szostak JW
Science. 1991;251(5001):1605-8 - PMID: 1707185

A poly(dA.dT) tract is a component of the recombination initiation site at the ARG4 locus in Saccharomyces cerevisiae.

Schultes NP, Szostak JW
Mol Cell Biol. 1991;11(1):322-8 - PMID: 1986228 - PMCID: PMC359622

Saccharomyces cerevisiae homoserine kinase is homologous to prokaryotic homoserine kinases.

Schultes NP, Ellington AD, Cherry JM, Szostak JW
Gene. 1990;96(2):177-80 - PMID: 2176637

Decreasing gradients of gene conversion on both sides of the initiation site for meiotic recombination at the ARG4 locus in yeast.

Schultes NP, Szostak JW
Genetics. 1990;126(4):813-22 - PMID: 1981763 - PMCID: PMC1204280

Chemical synthesis of oligoribonucleotides containing 2-aminopurine: substrates for the investigation of ribozyme function.

Doudna JA, Szostak JW, Rich A, Usman N
J Org Chem. 1990;55(21):5547-9 - PMID: 11540922

Mutational analysis of conserved nucleotides in a self-splicing group I intron.

Couture S, Ellington AD, Gerber AS, Cherry JM, Doudna JA, Green R, Hanna M, Pace U, Rajagopal J, Szostak JW
J Mol Biol. 1990;215(3):345-58 - PMID: 1700131

Phylogenetic and genetic evidence for base-triples in the catalytic domain of group I introns.

Michel F, Ellington AD, Couture S, Szostak JW
Nature. 1990;347(6293):578-80 - PMID: 2215683 - DOI: 10.1038/347578a0

Detection of heteroduplex DNA molecules among the products of Saccharomyces cerevisiae meiosis.

Lichten M, Goyon C, Schultes NP, Treco D, Szostak JW, Haber JE, Nicolas A
Proc Natl Acad Sci U S A. 1990;87(19):7653-7 - PMID: 2217196 - PMCID: PMC54806

Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML.

Park EC, Szostak JW
Mol Cell Biol. 1990;10(9):4932-4 - PMID: 2117703 - PMCID: PMC361112

In vitro genetic analysis of the Tetrahymena self-splicing intron.

Green R, Ellington AD, Szostak JW
Nature. 1990;347(6291):406-8 - PMID: 2215650 - DOI: 10.1038/347406a0

In vitro selection of RNA molecules that bind specific ligands.

Ellington AD, Szostak JW
Nature. 1990;346(6287):818-22 - PMID: 1697402 - DOI: 10.1038/346818a0

Miniribozymes, small derivatives of the sunY intron, are catalytically active.

Doudna JA, Szostak JW
Mol Cell Biol. 1989;9(12):5480-3 - PMID: 2685567 - PMCID: PMC363717

The guanosine binding site of the Tetrahymena ribozyme.

Michel F, Hanna M, Green R, Bartel DP, Szostak JW
Nature. 1989;342(6248):391-5 - PMID: 2685606 - DOI: 10.1038/342391a0

RNA structure, not sequence, determines the 5' splice-site specificity of a group I intron.

Doudna JA, Cormack BP, Szostak JW
Proc Natl Acad Sci U S A. 1989;86(19):7402-6 - PMID: 2678103 - PMCID: PMC298070

RNA-catalysed synthesis of complementary-strand RNA.

Doudna JA, Szostak JW
Nature. 1989;339(6225):519-22 - PMID: 2660003 - DOI: 10.1038/339519a0

A mutant with a defect in telomere elongation leads to senescence in yeast.

Lundblad V, Szostak JW
Cell. 1989;57(4):633-43 - PMID: 2655926

Stereochemical course of catalysis by the Tetrahymena ribozyme.

Rajagopal J, Doudna JA, Szostak JW
Science. 1989;244(4905):692-4 - PMID: 2470151

An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiae.

Nicolas A, Treco D, Schultes NP, Szostak JW
Nature. 1989;338(6210):35-9 - PMID: 2537472 - DOI: 10.1038/338035a0

Double-strand breaks at an initiation site for meiotic gene conversion.

Sun H, Treco D, Schultes NP, Szostak JW
Nature. 1989;338(6210):87-90 - PMID: 2645528 - DOI: 10.1038/338087a0

Telomerase: the beginning of the ends.

Szostak JW
Nature. 1989;337(6205):303-4 - PMID: 2765013 - DOI: 10.1038/337303a0

Gene conversion adjacent to regions of double-strand break repair.

Orr-Weaver TL, Nicolas A, Szostak JW
Mol Cell Biol. 1988;8(12):5292-8 - PMID: 3072478 - PMCID: PMC365631

Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae.

Murray AW, Claus TE, Szostak JW
Mol Cell Biol. 1988;8(11):4642-50 - PMID: 3062364 - PMCID: PMC365553

Genetic dissection of an RNA enzyme.

Doudna JA, Gerber AS, Cherry JM, Szostak JW
Cold Spring Harb Symp Quant Biol. 1988;52:173-80 - PMID: 2456876

Telomeric repeat from T. thermophila cross hybridizes with human telomeres.

Allshire RC, Gosden JR, Cross SH, Cranston G, Rout D, Sugawara N, Szostak JW, Fantes PA, Hastie ND
Nature. 1988;332(6165):656-9 - PMID: 2833706 - DOI: 10.1038/332656a0

Identification of healed terminal DNA fragments in linear minichromosomes of Schizosaccharomyces pombe.

Matsumoto T, Fukui K, Niwa O, Sugawara N, Szostak JW, Yanagida M
Mol Cell Biol. 1987;7(12):4424-30 - PMID: 2830493 - PMCID: PMC368126

Artificial chromosomes.

Murray AW, Szostak JW
Sci Am. 1987;257(5):62-8 - PMID: 3317814

Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae.

Reynolds AE, Murray AW, Szostak JW
Mol Cell Biol. 1987;7(10):3566-73 - PMID: 3316982 - PMCID: PMC368010

The yeast ARD1 gene product is required for repression of cryptic mating-type information at the HML locus.

Whiteway M, Freedman R, Van Arsdell S, Szostak JW, Thorner J
Mol Cell Biol. 1987;7(10):3713-22 - PMID: 3316986 - PMCID: PMC368027

Structural conventions for group I introns.

Burke JM, Belfort M, Cech TR, Davies RW, Schweyen RJ, Shub DA, Szostak JW, Tabak HF
Nucleic Acids Res. 1987;15(18):7217-21 - PMID: 3658691 - PMCID: PMC306243

Chromosome segregation in mitosis and meiosis.

Murray AW, Szostak JW
Annu Rev Cell Biol. 1987;1:289-315 - PMID: 3916318 - DOI: 10.1146/annurev.cb.01.110185.001445

An alternative pathway for meiotic chromosome segregation in yeast.

Dawson DS, Murray AW, Szostak JW
Science. 1986;234(4777):713-7 - PMID: 3535068

Construction and behavior of circularly permuted and telocentric chromosomes in Saccharomyces cerevisiae.

Murray AW, Szostak JW
Mol Cell Biol. 1986;6(9):3166-72 - PMID: 3537731 - PMCID: PMC367052

Enzymatic activity of the conserved core of a group I self-splicing intron.

Szostak JW
Nature. 1986;322(6074):83-6 - PMID: 3014350 - DOI: 10.1038/322083a0

Chromosome length controls mitotic chromosome segregation in yeast.

Murray AW, Schultes NP, Szostak JW
Cell. 1986;45(4):529-36 - PMID: 3518944

The ARD1 gene of yeast functions in the switch between the mitotic cell cycle and alternative developmental pathways.

Whiteway M, Szostak JW
Cell. 1985;43(2 Pt 1):483-92 - PMID: 3907857

Fungal recombination.

Orr-Weaver TL, Szostak JW
Microbiol Rev. 1985;49(1):33-58 - PMID: 3884994 - PMCID: PMC373016

Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents.

Ruby SW, Szostak JW
Mol Cell Biol. 1985;5(1):75-84 - PMID: 3920512 - PMCID: PMC366680

Transfer of yeast telomeres to linear plasmids by recombination.

Dunn B, Szauter P, Pardue ML, Szostak JW
Cell. 1984;39(1):191-201 - PMID: 6091911

DNA sequences of telomeres maintained in yeast.

Shampay J, Szostak JW, Blackburn EH
Nature. 1984;310(5973):154-7 - PMID: 6330571

Construction of specific chromosomal rearrangements in yeast.

Sugawara N, Szostak JW
Methods Enzymol. 1983;101:269-78 - PMID: 6310329

Construction of artificial chromosomes in yeast.

Murray AW, Szostak JW
Nature. 1983;305(5931):189-93 - PMID: 6350893

Yeast vectors with negative selection.

Brown PA, Szostak JW
Methods Enzymol. 1983;101:278-90 - PMID: 6310330

Cloning regulated yeast genes from a pool of lacZ fusions.

Ruby SW, Szostak JW, Murray AW
Methods Enzymol. 1983;101:253-69 - PMID: 6310328

Genetic applications of yeast transformation with linear and gapped plasmids.

Orr-Weaver TL, Szostak JW, Rothstein RJ
Methods Enzymol. 1983;101:228-45 - PMID: 6310326

A rapid procedure for the construction of linear yeast plasmids.

Szostak JW
Methods Enzymol. 1983;101:245-52 - PMID: 6310327

Pedigree analysis of plasmid segregation in yeast.

Murray AW, Szostak JW
Cell. 1983;34(3):961-70 - PMID: 6354471

Recombination between sequences in nonhomologous positions.

Sugawara N, Szostak JW
Proc Natl Acad Sci U S A. 1983;80(18):5675-9 - PMID: 6351064 - PMCID: PMC384321

Replication and resolution of telomeres in yeast.

Szostak JW
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1187-94 - PMID: 6305577

Yeast recombination: the association between double-strand gap repair and crossing-over.

Orr-Weaver TL, Szostak JW
Proc Natl Acad Sci U S A. 1983;80(14):4417-21 - PMID: 6308623 - PMCID: PMC384049

The double-strand-break repair model for recombination.

Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW
Cell. 1983;33(1):25-35 - PMID: 6380756

Multiple, tandem plasmid integration in Saccharomyces cerevisiae.

Orr-Weaver TL, Szostak JW
Mol Cell Biol. 1983;3(4):747-9 - PMID: 6343844 - PMCID: PMC368590

Is there left-handed DNA at the ends of yeast chromosomes?

Walmsley RM, Szostak JW, Petes TD
Nature. 1983;302(5903):84-6 - PMID: 6338397

Cloning yeast telomeres on linear plasmid vectors.

Szostak JW, Blackburn EH
Cell. 1982;29(1):245-55 - PMID: 6286143

Yeast transformation: a model system for the study of recombination.

Orr-Weaver TL, Szostak JW, Rothstein RJ
Proc Natl Acad Sci U S A. 1981;78(10):6354-8 - PMID: 6273866 - PMCID: PMC349037

DNA sequence of a mutation in the leader region of the yeast iso-1-cytochrome c mRNA.

Stiles JI, Szostak JW, Young AT, Wu R, Consaul S, Sherman F
Cell. 1981;25(1):277-84 - PMID: 6268305

Hybridization with synthetic oligonucleotides.

Szostak JW, Stiles JI, Tye BK, Chiu P, Sherman F, Wu R
Methods Enzymol. 1980;68:419-28 - PMID: 161605

Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae.

Szostak JW, Wu R
Nature. 1980;284(5755):426-30 - PMID: 6987539

Insertion of a genetic marker into the ribosomal DNA of yeast.

Szostak JW, Wu R
Plasmid. 1979;2(4):536-54 - PMID: 394173

Specific binding of a synthetic oligodeoxyribonucleotide to yeast cytochrome c mRNA.

Szostak JW, Stiles JI, Bahl CP, Wu R
Nature. 1977;265(5589):61-3 - PMID: 189200