Map created at http://pga.mgh.harvard.edu/web_apps/web_map/start Editor at http://pga.mgh.harvard.edu/web_apps/dna_utilities.html
*** Sites protected by E. coli methylation are not shown in this map *** Eco57 | GGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGA TCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCT 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGA Eco57 AlwN1 | BpuE1 | | | GAAGCCAGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGA 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CTTCGGTCAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACT ApaL1 BseY1 | | ACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAG 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 TGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTC Eci1 BciV1 BssS1 | | | GGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACA Pme1 Drd1 BpuE1 Eci1 Dra1 | | | | CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCAAGCTAGAGTTTAAAC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGTTCGATCTCAAATTTG Ssp1 | TTGACAGATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAAAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 AACTGTCTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTTTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAA amp > M S I Q H F R V A L I P F F Ale1 | GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCAGAAGATCACTTGGGTGCGCGAGTGGGTTACATCGAACTGG 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGTCTTCTAGTGAACCCACGCGCTCACCCAATGTAGCTTGACC amp > A A F C L P V F A H P E T L V K V K D A E D H L G A R V G Y I E L D Acl1 BpuE1 | Dra1 | | | ATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTCCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 TAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAGGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGC amp > L N S G K I L E S F R P E E R F P M M S T F K V L L C G A V L S R Bcg1b Bcg1a | | TATTGATGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAATACTCACCAGTCACAGAAAAGCATCTTACGGATGGC 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 ATAACTACGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTTATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCG amp > I D A G Q E Q L G R R I H Y S Q N D L V E Y S P V T E K H L T D G Bts1 Bts1 | | ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCG 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 TACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGATAGCCTCCTGGCTTCCTCGATTGGC amp > M T V R E L C S A A I T M S D N T A A N L L L T T I G G P K E L T A CTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCC 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 GAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGG amp > F L H N M G D H V T R L D R W E P E L N E A I P N D E R D T T M P BsrD1 Acl1 Eci1 | | | TGTAGCAATGGCAACAACGTTGCGAAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAACTAATAGACTGGATGGAGGCGGATAAAGTT 1201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1300 ACATCGTTACCGTTGTTGCAACGCTTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTGATTATCTGACCTACCTCCGCCTATTTCAA amp > V A M A T T L R K L L T G E L L T L A S R Q Q L I D W M E A D K V BsrD1 | GCAGGACCACTTCTGCGCTCGGCACTTCCGGCTGGCTGGTTTATTGCTGATAAATCAGGAGCCGGTGAGCGTGGGTCACGCGGTATCATTGCAGCACTGG 1301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1400 CGTCCTGGTGAAGACGCGAGCCGTGAAGGCCGACCGACCAAATAACGACTATTTAGTCCTCGGCCACTCGCACCCAGTGCGCCATAGTAACGTCGTGACC amp > A G P L L R S A L P A G W F I A D K S G A G E R G S R G I I A A L G Bmr1 | GGCCGGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACTACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTC 1401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1500 CCGGCCTACCATTCGGGAGGGCATAGCATCAATAGATGTGATGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAG amp > P D G K P S R I V V I Y T T G S Q A T M D E R N R Q I A E I G A S BsaXa BsaXb BciV1 Nde1 | | | | ACTGATTAAGCATTGGTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGT 1501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1600 TGACTAATTCGTAACCATTCCTATTTAAAGACCATTCCTCCTGTGCATACCTTCACCCGTTCAACCCCTTCGGCATAGGCAACGACTTAGACCGTATACA amp > L I K H W * Spe1 | GGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGGCATAACTAGTGAGGCTC 1601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1700 CCCTCATATTCTGCGCGTCGCAGCGTAGTCCGTAAAAAAAGACGCGGTTACGTTTTTCCGGTAGGCAGTCCTACCGGAAAGCCGTATTGATCACTCCGAG Mfe1 Age1 EcoN1 | | | CGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGG 1701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1800 GCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCC Bsg1 Bmr1 Eci1 Acl1 | | | | | GTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTC 1801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1900 CATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAG Sac2 Xmn1 | | GCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTC 1901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2000 CGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAG orf 2 < * K Pst1 Stu1 Afl2 BssS1 | | | | CACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCT 2001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2100 GTGGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGA orf 2 < W R A A T R S E Q D R A E P Q F H T P S N S A K R K L L G K A E H BseY1 BpuE1 | Dra1 | | | TGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAA 2101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2200 ACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTT orf 2 < K L Q P R A Q A S P G G R A F R T A G E R R D R Q K R Y T E L W K F BspM1 BfrB1 Bsg1 | | ATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGACGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGC 2201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2300 TAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTGCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCG orf 2 < N K I V Q Q S A K K R A L Y D Q L H P G L R D A C Q Y K P K Q P R Apa1 Sac2 PspOM | BseY1 EcoK Nsi1 BpuE1 | | | | | | | GGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGCATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGC 2301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2400 CCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCG orf 2 < P R R R P G H T G A C M C T R R P P A Q S R P W R S D S P P L R L Fse1 Nae1 | BseY1 Sac1 | | | | TGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAG 2401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2500 ACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTC orf 2 < S A P R S Q H R A E R R R T D G R G P R C P Q S S L I S S A A S P L BsrB1 Bpu10 BbvC1 Nar1 Bgl1 | | | | | AGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCT 2501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2600 TCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGA orf 2 < A P P H T V W V F S F P R E T R L R R K M Xho1 Dra3 | | CGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTT 2601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2700 GCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAA Bgl1Eco57 BpuE1 | | | AGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTT 2701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2800 TCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAA Xba1 Xmn1 | Hind3| | Xho1 || | | TTCTTCCATTTCAGGTGTCGTGAAAAGCTTCTAGAGATCCCTCGACCTCGAGATCCATTGTGCTCTAAAGGAGATACCCGGCCAGACACCCTCACCTGCG 2801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2900 AAGAAGGTAAAGTCCACAGCACTTTTCGAAGATCTCTAGGGAGCTGGAGCTCTAGGTAACACGAGATTTCCTCTATGGGCCGGTCTGTGGGAGTGGACGC Pvu2 BseY1 | BspM1| | BstX1 BspM1 BfrB1| | BbvC1 Bpu10 Nco1| Bsa1 Msc1 BseY1BfrB1 || | | | || | | | | GTGCCCAGCTGCCCAGGCTGAGGCAAGAGAAGGCCAGAAACCATGCCCATGGGGTCTCTGCAACCGCTGGCCACCTTGTACCTGCTGGGGATGCTGGTCG 2901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3000 CACGGGTCGACGGGTCCGACTCCGTTCTCTTCCGGTCTTTGGTACGGGTACCCCAGAGACGTTGGCGACCGGTGGAACATGGACGACCCCTACGACCAGC ENV > M P M G S L Q P L A T L Y L L G M L V A Nhe1 | CTTCCGTgctagccactgagaagctgtgggtgactgtgtactatggggtgcctgtgtggaaggaggccaccaccaccctgttctgtgcctctgatgccaa 3001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3100 GAAGGCAcgatcggtgactcttcgacacccactgacacatgataccccacggacacaccttcctccggtggtggtgggacaagacacggagactacggtt ENV > S V L A T E K L W V T V Y Y G V P V W K E A T T T L F C A S D A K ApaL1 Stu1 Ale1 | Dra3 Bgl1 | | | | | ggcctatgacactgaggtgcacaatgtgtgggccacccaggcctgtgtgcccactgaccccaacccccaggaggtggagctggtgaatgtgactgagaac 3101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3200 ccggatactgtgactccacgtgttacacacccggtgggtccggacacacgggtgactggggttgggggtcctccacctcgaccacttacactgactcttg ENV > A Y D T E V H N V W A T Q A C V P T D P N P Q E V E L V N V T E N BspLU Nsi1 AlwN1 Eco57 Bsg1 | | | | | ttcaacatgtggaagaacaacatggtggagcagatgcatgaggacatcatcagcctgtgggaccagagcctgaagccctgtgtgaagctgacccccctgt 3201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3300 aagttgtacaccttcttgttgtaccacctcgtctacgtactcctgtagtagtcggacaccctggtctcggacttcgggacacacttcgactggggggaca ENV > F N M W K N N M V E Q M H E D I I S L W D Q S L K P C V K L T P L C Bsu36 Bts1 | | gtgtgaccctgaactgcactgacctgaggaacaccaccaacaccaacaacagcactgccaacaacaacagcaactctgagggcaccatcaagggggggga 3301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3400 cacactgggacttgacgtgactggactccttgtggtggttgtggttgttgtcgtgacggttgttgttgtcgttgagactcccgtggtagttcccccccct ENV > V T L N C T D L R N T T N T N N S T A N N N S N S E G T I K G G E Pst1 BsrG1 | | gatgaagaactgcagcttcaacatcaccaccagcatcagggacaagatgcagaaggagtatgccctgctgtacaagctggacattgtgagcattgacaat 3401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3500 ctacttcttgacgtcgaagttgtagtggtggtcgtagtccctgttctacgtcttcctcatacgggacgacatgttcgacctgtaacactcgtaactgtta ENV > M K N C S F N I T T S I R D K M Q K E Y A L L Y K L D I V S I D N Pvu2 Ale1 Ale1 | | | gacagcaccagctacaggctgatcagctgcaacacctctgtgatcacccaggcctgccccaagatcagctttgagcccatccccatccactactgtgccc 3501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3600 ctgtcgtggtcgatgtccgactagtcgacgttgtggagacactagtgggtccggacggggttctagtcgaaactcgggtaggggtaggtgatgacacggg ENV > D S T S Y R L I S C N T S V I T Q A C P K I S F E P I P I H Y C A P Bts1 Stu1 BsrD1 Eco57 Pvu2 ApaL1 | Bsg1 | | | | | | | | ctgctggctttgccatcctgaagtgcaatgacaagaagttctctggcaagggcagctgcaagaatgtgagcactgtgcagtgcactcatggcatcaggcc 3601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3700 gacgaccgaaacggtaggacttcacgttactgttcttcaagagaccgttcccgtcgacgttcttacactcgtgacacgtcacgtgagtaccgtagtccgg ENV > A G F A I L K C N D K K F S G K G S C K N V S T V Q C T H G I R P AlwN1 Pvu2 | BbvC1 BseR1 BseY1 | | EcoN1| Bpu10 BseR1 | ApaL1 | | | || | | | | tgtggtgagcacccagctgctgctgaatggcagcctggctgaggaggaggtggtgatcaggtctgagaacttcactgacaatgccaagaccatcattgtg 3701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3800 acaccactcgtgggtcgacgacgacttaccgtcggaccgactcctcctccaccactagtccagactcttgaagtgactgttacggttctggtagtaacac ENV > V V S T Q L L L N G S L A E E E V V I R S E N F T D N A K T I I V Bsg1 Bsg1 Ear1 | | | cacctgaatgagtctgtgcagatcaactgcaccaggcccaactacaacaagaggaagaggattcacattggccctggcagggccttctacaccaccaaga 3801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3900 gtggacttactcagacacgtctagttgacgtggtccgggttgatgttgttctccttctcctaagtgtaaccgggaccgtcccggaagatgtggtggttct ENV > H L N E S V Q I N C T R P N Y N K R K R I H I G P G R A F Y T T K N Bts1 Bsu36 | | acatcattggcaccatcaggcaggcccactgcaacatcagcagggccaagtggaatgacaccctgaggcagattgtgagcaagctgaaggagcagttcaa 3901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4000 tgtagtaaccgtggtagtccgtccgggtgacgttgtagtcgtcccggttcaccttactgtgggactccgtctaacactcgttcgacttcctcgtcaagtt ENV > I I G T I R Q A H C N I S R A K W N D T L R Q I V S K L K E Q F K Sac1 Eco57 BstX1 SanD1 | | | gaacaagaccattgtgttcaaccagagctctgggggggaccctgagattgtgatgcacagcttcaactgtgggggggagttcttctactgcaacaccagc 4001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4100 cttgttctggtaacacaagttggtctcgagaccccccctgggactctaacactacgtgtcgaagttgacaccccccctcaagaagatgacgttgtggtcg ENV > N K T I V F N Q S S G G D P E I V M H S F N C G G E F F Y C N T S Pst1 Bts1 | | cccctgttcaacagcacctggaatggcaacaacacctggaacaacaccactggcagcaacaacaacatcaccctgcagtgcaagatcaagcagatcatca 4101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4200 ggggacaagttgtcgtggaccttaccgttgttgtggaccttgttgtggtgaccgtcgttgttgttgtagtgggacgtcacgttctagttcgtctagtagt ENV > P L F N S T W N G N N T W N N T T G S N N N I T L Q C K I K Q I I N BspLU Bsg1 | | acatgtggcaggaggtgggcaaggccatgtatgccccccccattgagggccagatcaggtgcagcagcaacatcactggcctgctgctgaccagggatgg 4201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4300 tgtacaccgtcctccacccgttccggtacatacggggggggtaactcccggtctagtccacgtcgtcgttgtagtgaccggacgacgactggtccctacc ENV > M W Q E V G K A M Y A P P I E G Q I R C S S N I T G L L L T R D G BsrG1 Bpm1 | | gggcaaggacactgacaccaatgacactgagattttcaggcctggggggggggacatgagggacaactggaggtctgagctgtacaagtacaaggtggtg 4301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4400 cccgttcctgtgactgtggttactgtgactctaaaagtccggacccccccccctgtactccctgttgacctccagactcgacatgttcatgttccaccac ENV > G K D T D T N D T E I F R P G G G D M R D N W R S E L Y K Y K V V Apa1 Ear1 PspOM | Xcm1 BseR1| Bsg1 Bgl1| | | || | || | actattgagcccctgggggtggcccccaccaaggccaagaggagggtggtgcagagggagaagagggctgccattggggccctgttcctgggcttcctgg 4401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4500 tgataactcggggacccccaccgggggtggttccggttctcctcccaccacgtctccctcttctcccgacggtaaccccgggacaaggacccgaaggacc ENV > T I E P L G V A P T K A K R R V V Q R E K R A A I G A L F L G F L G Bsg1 Bsg1 BbvC1 | | gggctgctggcagcactatgggggctgcctctgtgaccctgactgtgcaggccaggctgctgctgtctggcattgtgcagcagcagaacaacctgctgag 4501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4600 cccgacgaccgtcgtgatacccccgacggagacactgggactgacacgtccggtccgacgacgacagaccgtaacacgtcgtcgtcttgttggacgactc ENV > A A G S T M G A A S V T L T V Q A R L L L S G I V Q Q Q N N L L R Bpu10 BseY1 BspM1 | Sfi1 | BstAP Pvu2 Kpn1 BfrB1 | Bgl1 | BstAP | Pst1| Pvu2 Pst1 Acc65 | | | | | | | || | | | | ggccattgaggcccagcagcacatgctgcagctgactgtgtggggcatcaagcagctgcaggccagggtgctggctgtggagaggtacctgaaggaccag 4601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4700 ccggtaactccgggtcgtcgtgtacgacgtcgactgacacaccccgtagttcgtcgacgtccggtcccacgaccgacacctctccatggacttcctggtc ENV > A I E A Q Q H M L Q L T V W G I K Q L Q A R V L A V E R Y L K D Q Eco57 BseY1 | AlwN1 | Pvu2 | | Pvu2 AlwN1 | | Bsg1 Ale1 Bsm1 | Bpm1 | | | | | | | | cagctgctgggcttctggggctgctctggcaagctgatctgcaccaccactgtgccctggaatgccagctggagcaacaagagcctggatgacatctgga 4701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4800 gtcgacgacccgaagaccccgacgagaccgttcgactagacgtggtggtgacacgggaccttacggtcgacctcgttgttctcggacctactgtagacct ENV > Q L L G F W G C S G K L I C T T T V P W N A S W S N K S L D D I W N Sap1 Bts1 Ear1 BseY1 Bpm1 | | | | acaacatgacctggatgcagtgggagagggagattgacaactacaccagcctgatctacagcctgctggagaagagccagacccagcaggagaagaatga 4801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4900 tgttgtactggacctacgtcaccctctccctctaactgttgatgtggtcggactagatgtcggacgacctcttctcggtctgggtcgtcctcttcttact ENV > N M T W M Q W E R E I D N Y T S L I Y S L L E K S Q T Q Q E K N E BstAP PflM1 AlwN1 Bpm1 | BspH1 | | | | gcaggagctgctggagctggacaagtgggccagcctgtggaactggtttgacatcaccaactggctgtggtacatcaaaatcttcatcatgattgtgggg 4901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5000 cgtcctcgacgacctcgacctgttcacccggtcggacaccttgaccaaactgtagtggttgaccgacaccatgtagttttagaagtagtactaacacccc ENV > Q E L L E L D K W A S L W N W F D I T N W L W Y I K I F I M I V G BsmB1 Btr1 Bgl1Bsu36 Blp1| BmgB1BamH1 Bmr1 | | || | | | ggcctggtgggcctgaggattgtgtttgctgtgctgagctgagacgtgaggatccGAGGGTGAGTGCTCCTGCCTGGACGCATCCCGGCTATGCAGCCCC 5001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5100 ccggaccacccggactcctaacacaaacgacacgactcgactctgcactcctaggCTCCCACTCACGAGGACGGACCTGCGTAGGGCCGATACGTCGGGG ENV > G L V G L R I V F A V L S * orf 4 > M Q P Q Bpu10 Ear1 Bbs1 | | | | AGTCCAGGGCAGCAAGGCAGGCCCCGTCTGCCTCTTCACCCGGAGCCTCTGCCCGCCCCACTCATGCTCAGGGAGAGGGTCTTCTGGCTTTTTCCCAGGC 5101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5200 TCAGGTCCCGTCGTTCCGTCCGGGGCAGACGGAGAAGTGGGCCTCGGAGACGGGCGGGGTGAGTACGAGTCCCTCTCCCAGAAGACCGAAAAAGGGTCCG orf 4 > S R A A R Q A P S A S S P G A S A R P T H A Q G E G L L A F S Q A BspM1 BseY1 BspM1 Bsg1 EcoN1 BfrB1 AlwN1 BfrB1 | | | | | TCTGGGCAGGCACAGGCTAGGTGCCCCTAACCCAGGCCCTGCACACAAAGGGGCAGGTGCTGGGCTCAGACCTGCCAAGAGCCATATCCGGGAGGACCCT 5201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5300 AGACCCGTCCGTGTCCGATCCACGGGGATTGGGTCCGGGACGTGTGTTTCCCCGTCCACGACCCGAGTCTGGACGGTTCTCGGTATAGGCCCTCCTGGGA orf 4 > L G R H R L G A P N P G P A H K G A G A G L R P A K S H I R E D P BsaXb Bpu10 Bpu10 BbvC1 | BseR1 BsaXa | | | | | | GCCCCTGACCTAAGCCCACCCCAAAGGCCAAACTCTCCACTCCCTCAGCTCGGACACCTTCTCTCCTCCCAGATTCCAGTAACTCCCAATCTTCTCTCTG 5301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5400 CGGGGACTGGATTCGGGTGGGGTTTCCGGTTTGAGAGGTGAGGGAGTCGAGCCTGTGGAAGAGAGGAGGGTCTAAGGTCATTGAGGGTTAGAAGAGAGAC orf 4 > A P D L S P P Q R P N S P L P Q L G H L L S S Q I P V T P N L L S A Pst1 Bpm1 BpuE1 | | | CAGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGGTAAGCCAGCCCAGGCCTCGCCCTCCAGCTCAAGGCGGGACAGGTGCCCTAG 5401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5500 GTCTCGGGTTTAGAACACTGTTTTGAGTGTGTACGGGTGGCACGGGTCCATTCGGTCGGGTCCGGAGCGGGAGGTCGAGTTCCGCCCTGTCCACGGGATC orf 4 > E P K S C D K T H T C P P C P G K P A Q A S P S S S R R D R C P R Ear1 BbvC1 Ahd1 Btr1 BsaXb | BsaXa | BseY1 BmgB1 Bpu10 | | AlwN1 Bbs1 | | | | | | | | | | | AGTAGCCTGCATCCAGGGACAGGCCCCAGCCGGGTGCTGACACGTCCACCTCCATCTCTTCCTCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT 5501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5600 TCATCGGACGTAGGTCCCTGTCCGGGGTCGGCCCACGACTGTGCAGGTGGAGGTAGAGAAGGAGTCGTGGACTTGAGGACCCCCCTGGCAGTCAGAAGGA orf 4 > V A C I Q G Q A P A G C * Drd1 Btr1 Bsu36 | Ear1 Bsu36 Ale1 BmgB1 Bbs1 | | | | | | | CTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTC 5601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5700 GAAGGGGGGTTTTGGGTTCCTGTGGGAGTACTAGAGGGCCTGGGGACTCCAGTGTACGCACCACCACCTGCACTCGGTGCTTCTGGGACTCCAGTTCAAG orf 5 > M I S R T P E V T C V V V D V S H E D P E V K F BsaXa Sac2 BseR1 BsaXb Bsg1 | | | | | AACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCC 5701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5800 TTGACCATGCACCTGCCGCACCTCCACGTATTACGGTTCTGTTTCGGCGCCCTCCTCGTCATGTTGTCGTGCATGGCCCACCAGTCGCAGGAGTGGCAGG orf 5 > N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L BsaXb EcoN1 BsaXa Bsa1| BseY1 | | || | TGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGTGG 5801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5900 ACGTGGTCCTGACCGACTTACCGTTCCTCATGTTCACGTTCCAGAGGTTGTTTCGGGAGGGTCGGGGGTAGCTCTTTTGGTAGAGGTTTCGGTTTCCACC o