The DNA Sequencing Core at the Massachusetts General Hospital, located at 38 Sidney Street in Cambridge, functions both as a small-scale sequencing facility and a high-throughput center for large-scale sequencing projects. In order to accomodate the needs of the MGH community, there are four staff members who maintain operations seven days a week.

The facility is equipped with two ABI Prism 3730 DNA analyzers from Applied Biosystems, two PTC-225 thermal cycler DNA engines from MJ Research and one Hybaid multiblock system. The facility also includes a Qiagen Biorobot 3000 DNA purification station and a HiGro from Genemachines, functioning as an oxygenated culture incubation shaker. The Biorobot 3000 DNA purification station is capable of processing 800 samples per day.

The Center is capable of sequencing and analyzing thousands of samples each day with the 3730 DNA sequencers. With the introduction of a new automated submission system, we are capable of providing a turnaround time of 24 to 48 hours for final sequencing data once samples are received. The MGH Sequencing Core is a facility that serves the sequencing needs of molecular biology, the MGH-BWH Partners community, and outside academic and biotech institutions.

For more information, click on the following link: https://dnacore.mgh.harvard.edu/sequencing/index.shtml